High-level expression of a fungal pyranose oxidase in high cell-density fed-batch cultivations of Escherichia coli using lactose as inducer

0107921 - MBU-M 20040127 RIV NL eng J - Journal Article
Kotík, Michael - Kočanová, Marcela - Marešová, Helena - Kyslík, Pavel
High-level expression of a fungal pyranose oxidase in high cell-density fed-batch cultivations of Escherichia coli using lactose as inducer.
[Vysoká úroveň exprese houbové pyranosooxidasy při „fed-batch“ kultivacích Escherichia coli do vysoké density při použití laktosy jako induktoru.]
Protein Expression and Purification. Roč. 36, - (2004), s. 61-69. ISSN 1046-5928. E-ISSN 1096-0279
Institutional research plan: CEZ:AV0Z5020903
Keywords : fed-batch * lactose * inclusion bodies
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.336, year: 2004

Expression of a recombinant pyranose oxidase (P2O) from the basidiomycete Trametes ochracea in recombinant Escherichia coli strain was studied in batch and fed-batch cultivations. The P2O productivity has been improved using the fed-batch strategy and the optimized culture medium. The purified recombinant P2O was found to be similar to the P2O isolated from its original host.

Byla studována exprese rekombinantní pyranosooxidasy (P2O) z basidiomycety Trametes versicolor v rekombinantní Escherichia coli. Produktivita P2O byla zlepšeny při použití kultivace "fed batch" a optimalizací kultivačního média. Purifikovaná rekombinantní P2O se neliší PSO izolované z původního hostitele.
Permanent Link: http://hdl.handle.net/11104/0015061