Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings

0106530 - UACH-T 20043016 RIV GB eng J - Journal Article
Hynek, R. - Kučková, Štěpánka - Hradilová, J. - Kodíček, M.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a tool for fast identification of protein binders in color layers of paintings.
[Příprava nanokrystalického magnetitu vysokoenergetickým mletím železa a hematitu.]
Rapid Communications in Mass Spectrometry. Roč. 18, č. 17 (2004), s. 1896-1900. ISSN 0951-4198. E-ISSN 1097-0231
R&D Projects: GA ČR GA203/04/2091
Institutional research plan: CEZ:AV0Z4032918
Keywords : protein binders * mass spectrometry
Subject RIV: CA - Inorganic Chemistry
Impact factor: 2.750, year: 2004

Identification of materials in color layers of paintings is necessary for correct decisions concerning restoration procedures as well as proving the authenticity of the painting. The proteins are usually important components of the painting layers. In this paper it has been demonstrated that matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) can be used for fast and reliable identification of proteins in color layers even in old, highly aged matrices. The digestion can be easily performed directly on silica wafers which are routinely used for infrared analysis. The amount of material necessary for such an analysis is extremely small. Peptide mass mapping using digestion with trypsin followed by MALDI-TOFMS and identification of the protein was successfully used for determination of the binder from a painting of the 19th century.

Magnetit byl připraven mletím mikročástic hematitu a železa a pak studován chemickou analýzou, elektronovou mikroskopií, rtg difrakcí a Mossbauerovou spektroskopií. Magnetit se vyznačoval velkou magnetickou tvrdostí a po dlouhém mletí obsahoval příměs wustitu (FeO).
Permanent Link: http://hdl.handle.net/11104/0000215