Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells

0104352 - UEB-Q 20043015 RIV GB eng J - Journal Article
Mlejnek, P. - Doležel, Jaroslav - Procházka, S.
Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.
[Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.]
Plant Cell and Environment. Roč. 26, č. 10 (2003), s. 1723-1735. ISSN 0140-7791. E-ISSN 1365-3040
R&D Projects: GA MŠMT LN00A081
Institutional research plan: CEZ:AV0Z5038910
Keywords : adenosine 5'-triphosphate * cytokinin * N6-benzyladenine 9-riboside
Subject RIV: ED - Physiology
Impact factor: 3.613, year: 2003

Treatment of tobacco BY-2 cells with micromolar concentration of benzyladenosine ([9R]BA) resulted in the loss of cell viability in a time- and concentration-dependent manner. Cell death induced by [9R]BA exhibited typical apoptotic hallmarks including cell shrinkage, chromatin condensation and degradation of nuclear DNA to characteristic high molecular weight (HMW) as well as nucleosomal size fragments. Externally added [9R]BA was very rapidly and almost quantitatively phosphorylated within BY-2 cells. Accumulation of [9R]BA-monophosphate was accompanied by massive production of endogenous reactive oxygen species (ROS), intracellular ATP depletion, and these events were followed by the loss of cell viability. Inhibition of intracellular phosphorylation of [9R]BA by adenosin kinase inhibitor, 5'-amino-5'-deoxyadenosine (AdAs), diminished ROS production, ATP depletion, and consequently prevented cells from death.

Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.
Permanent Link: http://hdl.handle.net/11104/0011626