The influence of chemical structure of aliphatic polyesters on adhesion and growth of osteoblast-like MG63 cells

0104207 - FGU-C 20040150 RIV PL eng J - Journal Article
Pamula, E. - Bačáková, Lucie - Buczynska, J. - Filová, Elena - Nosková, Lenka - Dobrzynski, P. - Bero, M.
The influence of chemical structure of aliphatic polyesters on adhesion and growth of osteoblast-like MG63 cells.
[Vliv chemické struktury alifatických polyesterů na adhesi a růst osteoblastických buněk MG 63.]
Engineering of Biomaterials. Roč. 7, č. 37 (2004), s. 14-17. ISSN 1429-7248.
[Konferencja Naukowa Biomaterialy w medycynie i weterynarii /14./. Rytro, 10.10.2004-13.10.2004]
R&D Projects: GA MŠMT OC 527.130
Grant - others:Polish Committee for Scientific Research(PL) PBZ-KBN-082/T08/2002
Institutional research plan: CEZ:AV0Z5011922
Keywords : tissue engineering * degradable copolymers * physicochemical surface properties
Subject RIV: EI - Biotechnology ; Bionics

Degradable copolymers of glycolide and L-lactide (PGLA), glycolide and epsilon-caprolactone (PGCap) and terpolymer of glycolide, L-lactide and epsilon-caprolactone (PGLCap) were synthesized by ring opening polymerization and their interaction with osteoblast-like MG63 cells was tested in vitro. the cell behaviour on PGLA and PGLCap was very similar as on control tissue culture polystyrene (TCPS). On PGCap, however, the number of initially adhering cells was significantly lower (by 84% than on TCPS) and cell spreading area smaller (by 50%). On day 7 after seeding, these cells also reached the lowest populaton density. We hypothesize that the lower cell adhesion and growth of MG63 cells on PGCap was caused by the highest hydrophobicity of this material among all studied samples.

Na degradovatelných kopolymerech glykolidu a L-lactidu (PGLA), glykolidu a epsilon-kaprolaktonu (PGCap) a terpolymeru glykolidu, L-laktidu a epsilon-kaprolaktonu (PGLCap) byla sledována adhese a růst osteogenních buněk MG 63 in vitro. Počet i adhesní plocha buněk na PGLA and PGLCap byl podobný jako na standardním polystyrénovém kultivačním substrátu, zatímco na PGCap byl tento počet i plocha významně nižší (o 84% a 50%). Rovněž plocha rozprostření buněk na PGCap kontaktu bu nižší and cell spreading area smaller (by 50%). Navíc za 7 dní po nasazení dosáhly buňky na PGCap nejnižší populační density. Předpokládáme, že snížení adhese a růstu buněk na PGCap bylo způsobeno relativně vysokou hydrofobicitou tohoto materiálu
Permanent Link: http://hdl.handle.net/11104/0011485