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Cell proliferation on UV-excimer lamp modified and grafted polytetrafluoroethylene

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    0101871 - UJF-V 20043054 RIV CZ eng J - Journal Article
    Švorčík, V. - Ročková, K. - Ratajová, E. - Heitz, J. - Bauerle, D. - Bačáková, L. - Dvořánková, B. - Hnatowicz, Vladimír
    Cell proliferation on UV-excimer lamp modified and grafted polytetrafluoroethylene.
    [Proliferace buněk na modifikovaném UV zářením z excimerové lampy a roubováním.]
    Nuclear Instruments & Methods in Physics Research Section B. Roč. 217, č. 2 (2004), s. 307-313. ISSN 0168-583X. E-ISSN 1872-9584
    R&D Projects: GA ČR GA106/03/0514; GA AV ČR IAA5011301
    Institutional research plan: CEZ:AV0Z1048901
    Keywords : polytetraflujoroethylene * UV-beam modification in reactive atmosphere
    Subject RIV: BG - Nuclear, Atomic and Molecular Physics, Colliders
    Impact factor: 0.997, year: 2004

    Polytetrafluoroethylene (PTFE) was modified by irradiation with 172 nm light from an UV-excimer lamp in reactive ammonia atmosphere. Then, the samples were exposed to aqueous solutions of amino acids (glycine, alanine and leucine). The samples were characterized by water contact angle measurement, UV-VIS spectroscopy and laser-induced fluorescence (LIF). The results indicate, that the amino acids are bound onto the polymer chain. The amount polytetrafluoroethylenu of incorporated amino acid is a decreasing function of its molecular size. By in vitro experiments it was shown that the incorporated amino acids support adhesion and proliferation of rat aortic smooth muscle cells and mouse embryonic 3T3 fibroblasts on the polymer surface. The maximum effect was observed for smallest molecule of glycine

    Polytetrafluoroethylen (PTFE) byl modifikován UV zářením z excimerové lampy v reaktivní atmosféře amoniaku a roubován aminokyselinami (glycine, alanine a leucine). Pokusy in vitro bylo prokázáno, že inkorporované aminokyseliny podporují adhesi a proliferaci myších hladkých svalových buněk a 3T3 fibroblastů.
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