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Enzyme treatment for elimination of egg stickness in tench (Tinca Tinca L.), European catfish (Silurus Glanis L.) and common carp (Cyprinus Carpio L.)

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    0100609 - UZFG-Y 20043028 RIV NL eng J - Journal Article
    Linhart, Otomar - Rodina, Marek - Gela, David - Flajšhans, Martin - Kocour, Martin
    Enzyme treatment for elimination of egg stickness in tench (Tinca Tinca L.), European catfish (Silurus Glanis L.) and common carp (Cyprinus Carpio L.).
    [Enzymatické odlepování jiker lína obecného (Tinca Tinca L.), sumce velkého (Silurus Glanis L.) a kapra obecného (Cyprinus Carpio L.).]
    Fish Physiology and Biochemistry. Roč. 28, - (2004), s. 507-508. ISSN 0920-1742. E-ISSN 1573-5168
    R&D Projects: GA MŠMT ME 403; GA MŠMT ME 420; GA AV ČR IBS5045314
    Institutional research plan: CEZ:AV0Z5045916
    Keywords : aquaculture * egg * enzyme
    Subject RIV: ED - Physiology
    Impact factor: 0.399, year: 2004

    Enzyme treatment to eliminate egg stickness in tench, European catfish and common carp was compared with standard methodology in an attempt to decrease time consuming under hatchery conditions. Eggs of tench and E. catfish were exposed to an alcalase enzyme MERCK EC 3.4.21.14(0.6 AU g-1) solution 3 min after egg fertilization for 2 min. The best enzyme concentration in tench and E. catfish was 10 and 20 ml.l-1 of enzyme, respectively. The eggs of c.carp were successfully destickness with ALCALASE DX (2.5AU g-1) using two concentration of enzyme (2 ml.l-1 a 20 ml.l-1) from 8 to 20 min after fertilization.

    Enzymatické odlepkování jiker lína, sumce a kapra bylo porovnáváno s klasickou metodou s cílem zkrátit odlepkovámí v provozních podmínkách rybí líhně. Jikry lína a sumce byly odlepkovávány enzymem alkalázou MERCK EC 3.4.21.14 (0.6 AU g-1) v čase 3 min po aktivaci jiker po dobu 2 min. Nejlepší koncentrace enzymu byla pro lína a sumce 10 a 20 ml.l-1. Jikry kapra byly úspěšně odlepovány enzymem ALCALASE DX (2.5 AU g-1) při použití dvou koncentrací enzymu (2 ml.l-1 a 20 ml.l-1) od 8 do 20 min po aktivaci jiker.
    Permanent Link: http://hdl.handle.net/11104/0008107
     
Number of the records: 1  

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