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Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls

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    0099652 - ÚEB 2008 RIV NL eng J - Journal Article
    Gichner, Tomáš - Lovecká, P. - Kochánková, L. - Macková, M. - Demnerová, K.
    Monitoring toxicity, DNA damage, and somatic mutations in tobacco plants growing in soil heavily polluted with polychlorinated biphenyls.
    [Monitorování toxicity, poškození DNA a somatické mutace u rostlin tabáku rostoucí na půdě s vysokým obsahem polychlorovaných bifenylů.]
    Mutation Research - Genetic Toxicology and Environmental Mutagenesis. Roč. 629, č. 1 (2007), s. 1-6. ISSN 1383-5718. E-ISSN 1879-3592
    R&D Projects: GA ČR GA521/05/0500
    Grant - others:Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská(CZ) 1P05ME745; Vysoká škola chemicko-technologická v Praze / Fakulta chemicko-inženýrská(CZ) OC 117
    Institutional research plan: CEZ:AV0Z50380511
    Source of funding: V - Other public resources ; V - Other public resources ; V - Other public resources
    Keywords : comet assay * single-cell gel electrophoresis * gas chromatography
    Subject RIV: EB - Genetics ; Molecular Biology
    Impact factor: 2.278, year: 2007

    Heterozygous tobacco (Nicotiana tabacum var. xanthi) plants were cultivated in soil from a dump site highly polluted with polychlorinated biphenyls (PCBs) at Lhenice in South Bohemia, Czech Republic. The total amount of PCBs in the polluted soil, measured by gas chromatography varied from 165 to 265 mg kg(-1) of soil. In tobacco plants cultivated for 8 weeks in the polluted soil the amount of PCBs in the leaves varied from 11 to 28 and in the roots from 104 to 308 mg kg(-1) dry mass. The average leaf area of tobacco plants growing in the PCB-polluted soil was significantly reduced and the DNA damage in leaf nuclei, measured by the comet assay, was slightly but significantly increased compared with controls. The tobacco plants with increased DNA damage showed reduced growth and had distorted leaves. No increase in the frequency of somatic mutations was detected in tobacco plants growing in the PCB-polluted soil.

    Semenáčky heterozygotního tabáku Nicotiana tabacum kultivar. xanthi byly pěstovány po dobu 6 týdnů na půdě obsahující 165 až 265 mg PCB na kg-1. Poškození DNA u těchto rostlin, měřeno technikou komet, bylo průkazně zvýšeno s porovnání s kontrolou. Ke zvýšení somatických mutací v listech heterozygotního tabáku nedošlo.
    Permanent Link: http://hdl.handle.net/11104/0158203

     
     
Number of the records: 1  

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