Calcium responses to thyrotropin-releasing hormone and angiotensin II. The role of plasma membrane integrity and efect of G11-alpha protein overexpression on homologous and heterologous desensitization

0098855 - FGÚ 2008 CZ eng A - Abstract
Novotný st., Jiří - Ostašov, Pavel - Krůšek, Jan - Durchánková, Dana - Svoboda, Petr
Calcium responses to thyrotropin-releasing hormone and angiotensin II. The role of plasma membrane integrity and efect of G11-alpha protein overexpression on homologous and heterologous desensitization.
[Vapníková odpověď na thyroliberin a angiotensin II. Úloha integrity plasmatické membrány a efekt overexprese G11-alpha proteinu v homologní a heterologní desensitizaci.]
Programme and abstracts of the 6th Conference of the Czech Neuroscience Society. Praha: The Czech Neuroscience Society, 2007. s. 87-87.
[Conference of the Czech Neuroscience Society /6./. 19.11.2007-20.11.2007, Praha]
R&D Projects: GA AV ČR(CZ) IAA500110606; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z50110509
Keywords : spo2 * calcium response * cholesterol depletion * desensitization
Subject RIV: CE - Biochemistry

Here we analyzed hormone-induced Ca2+ responses and the process of desenzitization in HEK 293 cells, which express endogenous angiotensin II receptor. These cells were transfected to express also high levels of TRH receptors or both TRH receptors and G11alpha protein. It might be concluded that the level of expression of G-protein-coupled receptors and their cognate G-proteins strongly influences not only the magnitude of cellular response but also the process of desensitization and resilience of transmembrane signaling mediated by these receptors to changes in the plasma membrane structure caused by cholesterol depletion

V této práci jsme analyzovali Ca2+ odpovědi vyvolané hormonem a proces desenzitizace v buňkách HEK 293, které přirozeně exprimují receptor pro angiotensin II. Tyto buňky byly transfekovány pro expresi vysokých hladin TRH receptoru nebo TRH receptoru spolu s G11alpha proteinem. Z našich experimentů může být učiněn závěr, že úroveň exprese TRH receptoru a s ním spřaženého G proteinu výrazně ovlivňuje nejen velikost odpovědi, ale také proces desenzitizace a citlivost transmembránové signalizace zprostředkované těmito receptory na změny v integritě plasmatické membrány způsobené odstraněním cholesterolu
Permanent Link: http://hdl.handle.net/11104/0157661