Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells

0091554 - ÚMG 2008 RIV US eng J - Journal Article
Katsetos, C.D. - Dráberová, Eduarda - Šmejkalová, Barbora - Reddy, G. - Bertrand, L. - de Chadarévian, J.-P.
Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells.
[BetaIII-tubulin a gama-tubulin jsou koexprimovány a formují komplexy v lidských glioblastomech.]
Neurochemical Research. Roč. 32, č. 8 (2007), s. 1387-1398. ISSN 0364-3190. E-ISSN 1573-6903
R&D Projects: GA MŠMT LC545
Institutional research plan: CEZ:AV0Z50520514
Keywords : gamma-tubulin * class III beta-tubulin * centrosome amplification
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.811, year: 2007

We determined the expression, distribution and interaction of beta-III and gamma-tubulins in astrocytic gliomas and T98G glioblastoma cell line. In contrast with astrocytes, on T98G cells we found striking amount of gamma-tubulin in soluble cytoplasmic pool. Whereas both tubulins formed complexes in cytoplasm, beta-III did not associate with insoluble gamma-tubulin aggregates.

Byla porovnána distribuce, exprese a interakce beta-III a gama-tubulinu u astrocytů a lidské glioblastomové linie T98G. U T98G buněk byla pozorována vysoká exprese gama-tubulinu v cytoplazmě, kde vytváří komplexy s beta-III-tubulinem. Toto atypické chování obou proteinů odráží proces transformace gliových buněk.
Permanent Link: http://hdl.handle.net/11104/0152114