Functionalised magnetic nano- and microspheres for molecular diagnostic applications

0090453 - ÚMCH 2008 RIV HU eng C - Conference Paper (international conference)
Rittich, B. - Španová, A. - Horák, Daniel - Beneš, Milan J.
Functionalised magnetic nano- and microspheres for molecular diagnostic applications.
[Funkcionalizované magnetické nano- a mikročástice pro aplikace v molekulární diagnostice.]
Biopowders. Advances in the Powder Technology. Veszprém: Research Institute of Chemical and Process Enginnering, University of Pannonia, 2007 - (Feczkó, T.), s. 116-124. ISBN 978-963-9696-23-5.
[Biopowders Mini-Conference. Budapest (HU), 13.09.2007-14.09.2007]
R&D Projects: GA ČR GA203/05/2256
Grant - others:GA MZe(CZ) 1G57037
Program: 1G
Institutional research plan: CEZ:AV0Z40500505
Keywords : nonporous magnetic hydrophilic microspheres * P(HEMA-co-EDMA) * P(HEMA-co-GMA) * PGMA
Subject RIV: EB - Genetics ; Molecular Biology

Magnetic microspheres P(HEMA-co-EDMA), P(HEMA-co-GMA), and PGMA with hydrophilic properties were prepared by single-step dispersion polymerisation. Several enzymes, such as RNase A, DNase I, proteinase K and Salmonella antibodies were immobilised on the microspheres and their applicability in degradation of bacterial RNA, chromosomal and plasmid DNA, magnetic separation of Salmonella cells or degradation of their intracellular inhibitors was demonstrated.

Byly připraveny magnetické P(HEMA-co-EDMA), P(HEMA-co-GMA) a PGMA mikročástice dispersní polymerizací. Na mikročástice byla imobilizována řada enzymů, jako je RNasa A, DNasa I, proteináza K a protilátka proti Salmonele. Byly použity pro degradaci bakteriální RNA, chromozomální a plasmidové DNA, magnetickou separaci buněk Salmonely nebo degradaci jejich intracelulárních inhibitorů.
Permanent Link: http://hdl.handle.net/11104/0151340