Increased ventricular preload is compensated by myocyte proliferation in normal and hypoplastic fetal chick left ventricle

0090296 - ÚŽFG 2008 RIV US eng J - Journal Article
Dealmeida, A. - McQuinn, T. C. - Sedmera, David
Increased ventricular preload is compensated by myocyte proliferation in normal and hypoplastic fetal chick left ventricle.
[Zvýšené plnění komor je kompenzováno zvýšením proliferace myocytů v normálním i hypoplastické levé komoře.]
Circulation Research. Roč. 100, - (2007), s. 1363-1370. ISSN 0009-7330. E-ISSN 1524-4571
Institutional research plan: CEZ:AV0Z50450515
Keywords : chick embryo * hemodynamics * fetal surgery * hypoplastic left heart syndrome
Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery
Impact factor: 9.721, year: 2007

Hemodynamics influence cardiac development, and alterations in blood flow may lead to impaired cardiac growth and malformations. The developing myocardium adapts to augmented workload by increasing cell number (hyperplasia). The aim of this study was to determine the influence of alterations in ventricular preload on fetal myocyte proliferation by manipulation of intracardiac shunting at the atrial level. We hypothesized that partial clipping of the right atrial appendage would increase the blood flow to the left ventricle and, in turn, lead to an increase in chamber volume and myocardial mass based on myocyte proliferation. Using an ex ovo culture setup, we performed partial right atrial clipping on embryonic day 8 chick embryos. Ultrasound imaging was performed before and after the surgery to assess the changes in left ventricular volume. Sampling after 24 hours was preceded by 2 hour of pulse-labeling with 5-bromodeoxyuridine. Ultrasound imaging showed that partial right atrial clipping led to a significant increase in left ventricular end-diastolic volume, demonstrating increased blood flow and preload. Anti-5-bromodeoxyuridine immunolabeling revealed a significant increase in myocyte proliferation in the left ventricle and atrium. No significant changes were found in the right heart structures. Increased left ventricular myocyte proliferation and myocardial mass after right atrial clipping was also observed in embryos with experimental left ventricular hypoplasia. These results demonstrate the ability of fetal myocardium to respond to increased preload by myocyte hyperplasia and support the rationale for prenatal surgical interventions in certain cases of congenital heart disease such as hypoplastic left heart syndrome.

Práce popisuje zvýšení proliferace myocytů po fetálním chirucgickém zákroku na kuřecím zárodku v experimentálním modelu levostranné srdeční hypoplázie.
Permanent Link: http://hdl.handle.net/11104/0151229