On the chlorophyll a fluorescence yield in chloroplasts upon excitation wtih twin turnover flashes (TTF) and high frequency flash trains

0090151 - MBÚ 2008 RIV SIGLE NL eng J - Journal Article
Vredenberg, W. - Durchan, M. - Prášil, Ondřej
On the chlorophyll a fluorescence yield in chloroplasts upon excitation wtih twin turnover flashes (TTF) and high frequency flash trains.
[Výtěžek fluorescence chlorofylu po excitaci mnohoobrátkovými záblesky a při použití serie záblesků.]
Photosynthesis Research. Roč. 93, - (2007), s. 183-192. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA ČR GA206/05/0335
Institutional research plan: CEZ:AV0Z50200510; CEZ:AV0Z50510513
Keywords : chlorophyll a * fluorescence quenching * single turnover excitation
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.139, year: 2007

Chlorophyll fluorescence is routinely taken as a quantifiable measure of the redox state of the primary quinone acceptor QA of PSII. The variable fluorescence in thylakoids increases in a single turnover flash (STF) from its low dark level Fo towards a maximum FmSTF when QA becomes reduced. We found, using twin single turnover flashes (TTFs) that the fluorescence increase induced by the first twin-partner is followed by a 20 – 30% increase when the second partner is applied within 20 to 100 μs after the first one. The amplitude of the twin response shows a period-of-four oscillation associated with the 4-step oxidation of water in the Kok cycle (S states) and originates from two different trapped states with a life time of 0.2 to 0.4 and 2 to 5 ms, respectively

Práce se zabývá interpretací variabilní fluorescence chlorofylu, která se rutinně používá při studiu fotosyntézy. Nově popisuje možný mechanismus nárůstu variabilní fluorescence během serie krátkých záblesků a to pomocí aktivity tzv. Qb neredukujících center Fotosystému II
Permanent Link: http://hdl.handle.net/11104/0151144