Can microsatellite markers resolve phylogenetic relationships between closely related crested newt species (Triturus cristatus superspecies)?

0089593 - ÚBO 2008 RIV NL eng J - Journal Article
Mikulíček, P. - Crnobrnja-Isailović, J. - Piálek, Jaroslav
Can microsatellite markers resolve phylogenetic relationships between closely related crested newt species (Triturus cristatus superspecies)?
[Mohou mikrosatelity vyřešit fylogenetické vztahy mezi čolky skupiny Triturus cristatus superspecies?]
Amphibia-Reptilia. Roč. 28, č. 4 (2007), s. 467-474. ISSN 0173-5373. E-ISSN 1568-5381
R&D Projects: GA ČR GA206/01/0695; GA MŠMT LC06073
Institutional research plan: CEZ:AV0Z60930519
Keywords : crested newt * microsatelitte markers
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.929, year: 2007

Theoretical and empirical studies revealed that fast evolving microsatellite markers might be successfully employed in phylogenetic reconstruction. In this study we used seven highly polymorphic microsatellite loci and six different genetic distances to infer the performance of microsatellites in estimation of phylogenetic relationships between closely related crested newt species (Triturus cristatus superspecies). Many intraspecific distances exceeded interspecific values likely due to loss of genetic distance linearity in time, revealing that none of them was able to estimate divergence between the species. The Neighbor-joining trees constructed on the basis of genetic distances showed trichotomies (three-way polychotomies) and low bootstrap support at the species level. This study thus revealed limits of microsatellites to estimate phylogenetic relationships between the crested newt taxa.

Studie odhaluje limity mikrosatelitů při odhadování fylogenetických vztahů mezi druhy čolků Triturus cristatus superspecies.
Permanent Link: http://hdl.handle.net/11104/0150755