Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus

0088898 - MBÚ 2008 RIV SIGLE NL eng J - Journal Article
Simerská, Pavla - Monti, D. - Čechová, Ivana - Pelantová, Helena - Macková, M. - Bezouška, Karel - Riva, S. - Křen, Vladimír
Induction and characterization of an unusual Alfa-D-galactosidase from Talaromyces flavus.
[Indukce a charakterizace neobvyklé Alfa-D-galaktosidasy z Talaromyces flavus.]
Journal of Biotechnology. Roč. 128, č. 1 (2007), s. 61-71. ISSN 0168-1656. E-ISSN 1873-4863
R&D Projects: GA ČR GA203/05/0172; GA MŠMT OC D25.002; GA MŠMT LC06010; GA MŠMT OC D25.001
Grant - others:US(US) NATO LST.CLG.980125
Institutional research plan: CEZ:AV0Z50200510
Source of funding: N - Non-public resources
Keywords : alfa-d-galactosidase * talaromyces flavus * enzyme purification
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.565, year: 2007

An extracellular alfa-D-galactosidase from Talaromyces flavus CCF 2686 with extremely broad and unusual acceptor specificity is produced exclusively in the presence of the specific inducer - 6-deoxy-D-glucose (quinovose). The procedure for the preparation of this very expensive substance has been modified and optimized. Surprisingly, any of other common alfa-D-galactosidase inducers or substrates, e.g., D-galactose, melibiose and raffinose, did not stimulate its production. The crude alfa-D-galactosidase preparation was purified by anion-exchange chromatography and three isoenzymes with different substrate specificities were identified

Extracelulární alfa-D-galaktosidasa z Talaromyces flavus CCF 2686 s extrémě širokou a neobvyklou substrátovou specifitou je produkována výhradně v přítomnosti specifického induktoru - 6-deoxy-D-glukosy (quinovosa). Enzym byl purifikován aniontově-výměnnou chromatografií a byly identifikovány 3 isoenzymy s rozdílnou substrátovou specifitou
Permanent Link: http://hdl.handle.net/11104/0150279