Circadian rhythm gene regulation in the housefly, Musca domestica

0088766 - BC 2008 RIV US eng J - Journal Article
Codd, V. - Doležel, David - Stehlík, Jan - Piccin, A. - Garner, K. J. - Racey, S. N. - Straatman, K. R. - Louis, E. J. - Costa, R. - Šauman, Ivo - Kyriacou, C. P. - Rosato, E.
Circadian rhythm gene regulation in the housefly, Musca domestica.
[Regulace cirkadiánních rytmů u mouchy domácí (moucha domácí).]
Genetics. Roč. 177, č. 3 (2007), s. 1539-1551. ISSN 0016-6731. E-ISSN 1943-2631
R&D Projects: GA ČR GA204/04/0862; GA MŠMT 2B06129
Institutional research plan: CEZ:AV0Z50070508
Keywords : circadian * evolution * Diptera
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.001, year: 2007

The circadian mechanism appears remarkably conserved between Drosophila and mammals, with basic underlying negative and positive feedback loops, cycling gene products, and temporally regulated nuclear transport involving a few key proteins. One of these negative regulators is PERIOD, which in Drosophila shows very similar temporal and spatial regulation to TIMELESS. Surprisingly, we observe that in the housefly, Musca domestica, PER does not cycle in western blots of head extracts, in contrast to the TIM protein. Furthermore immunocytochemical (ICC) localization using enzymatic staining procedures, reveals that PER is not localized to the nucleus of any neurons within the brain at any circadian time, as recently observed for several nondipteran insects. However with confocal analysis, immunofluorescence reveals a very different picture, and provides an initial comparison of PER/TIM containing cells in Musca and Drosoph., which shows some significant differences, but many similarities.

Stanovili jsme množství mRNA cirkadiánních transkriptů mouchy domácí (Musca domestica), lokalizovali mRNA a proteiny a ukázali, že regulace cirkadiánních rytmů mouchy domací se liší od blízce příbuzné Drosophila melanogaster.
Permanent Link: http://hdl.handle.net/11104/0150187