Volume visualization of large biological tissue specimens captured by a confocal laser scanning microscope

0087376 - FGÚ 2008 RIV FR eng C - Conference Paper (international conference)
Čapek, Martin - Janáček, Jiří - Karen, Petr - Kubínová, Lucie - Hána, P. - Smrčka, P.
Volume visualization of large biological tissue specimens captured by a confocal laser scanning microscope.
[Objemová vizualizace obrazů velkých biologických tkáňových vzorků nasnímaných laserovým konfokálním mikroskopem.]
ICS´12. Saint-Etienne: International society for stereology, 2007, s. 12-17.
[International congress for stereology /12./. Saint-Etienne (FR), 03.09.2007-07.09.2007]
R&D Projects: GA MŠMT(CZ) LC06063; GA AV ČR(CZ) IAA100110502; GA AV ČR(CZ) IAA600110507; GA ČR(CZ) GA304/05/0153; GA AV ČR(CZ) IAA500200510
Institutional research plan: CEZ:AV0Z50110509
Keywords : confocal microscope * volume visualization
Subject RIV: JC - Computer Hardware ; Software

We apply volume reconstruction for visualization of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM). The first step of volume reconstruction is cutting large tissue specimens into thin physical slices. Second, volume image data sets (spatial tiles which overlap) are captured from all studied physical slices by CLSM. The third step is merging of overlapping spatial tiles of the same physical slice in horizontal direction (mosaicing). The fourth reconstruction step is merging of volumes of successive physical slices in vertical direction by applying an elastic registration algorithm. The last step is an image enhancement to compensate for the effect of the light attenuation with depth occurring in confocal microscopy. The resulting large digital volumes are visualized by a hardware accelerated volume rendering. In this paper we show a reconstruction of a middle part of a 21-days-old laboratory Norway rat embryo

Aplikujeme objemovou rekonstrukci na vizualizaci tkáňových preparátů větších než je zorné pole konfokálního laserového rastrovacího mikroskopu. Výsledný digitální objem vizualizujeme pomocí hardwarových prostředků. V tomto článku ukazujeme rekonstrukci na embryu laboratorní krysy o stáří 21 dnů
Permanent Link: http://hdl.handle.net/11104/0149234