LP-12 Approaches to visualization of 3D structures captured by a confocal microscope

0087268 - FGÚ 2008 RIV CZ eng C - Conference Paper (international conference)
Čapek, Martin - Janáček, Jiří - Kubínová, Lucie - Hána, K. - Smrčka, P.
LP-12 Approaches to visualization of 3D structures captured by a confocal microscope.
[Přístupy k visualizaci obrazů 3D struktur získaných konfokálním mikroskopem.]
Proceedings 8th multinational congress on microscopy. České Budějovice: Czechoslovak microscopy society, 2007 - (Nebesářová, J.; Hozák, P.), s. 453-454. ISBN 978-80-239-9397-4.
[Multinational Congress on Microscopy /8./. Prague (CZ), 17.06.2007-21.06.2007]
R&D Projects: GA AV ČR(CZ) IAA100110502; GA AV ČR(CZ) IAA500200510; GA ČR(CZ) GA304/05/0153; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z50110509
Keywords : confocal microscopy * volume visualization
Subject RIV: JC - Computer Hardware ; Software

Laser scanning confocal microscopes are capable to focus a laser beam into a layer of an investigated biological specimen, and by the gradual scanning of this layer they acquire an optical section. By consecutive scanning of all preset layers of the specimen we obtain a stack of optical sections, i.e. a 3D digital representation of the specimen. In the presented study we focus on volume reconstruction of large biological tissues, i.e. tissues greater than field of view and/or thicker than maximal depth of scanning of a confocal microscope. As a result of volume reconstruction we obtain a high resolution 3D image of the biological specimen. 3D visualization is offered either by our Rapid3D software package suited for three-dimensional reconstruction and visualization of biomedical images, or Ellipse modular software package devoted to biological image processing (created by ViDiTo company, Slovakia)

Článek popisuje přístupy k objemové rekonstrukci a vizualizaci 3D biologických struktur nasnímaných konfokálním mikroskopem
Permanent Link: http://hdl.handle.net/11104/0149164