Cocaine- and amphetamine-regulated transcript (CART) peptide specific binding in pheochromocytoma cells PC12

0084044 - ÚOCHB 2008 RIV NL eng J - Journal Article
Maletínská, Lenka - Maixnerová, Jana - Matyšková, Resha - Haugvicová, Renata - Šloncová, Eva - Elbert, Tomáš - Slaninová, Jiřina - Železná, Blanka
Cocaine- and amphetamine-regulated transcript (CART) peptide specific binding in pheochromocytoma cells PC12.
[Specifická vazba peptidu CART (cocaine- and amphetamine-regulated transcript) k pheochromocytomálním buňkám PC12.]
European Journal of Pharmacology. Roč. 559, 2/3 (2007), s. 109-114. ISSN 0014-2999. E-ISSN 1879-0712
R&D Projects: GA ČR GA303/05/0614
Institutional research plan: CEZ:AV0Z40550506; CEZ:AV0Z50520514; CEZ:AV0Z50200510
Keywords : radioligand binding * CART * PC12 cells * food intake
Subject RIV: CE - Biochemistry
Impact factor: 2.376, year: 2007

We report specific binding of 125I-CART(61-102) to the rat adrenal pheochromocytoma PC12 cell line, both intact cells and cell membranes. Saturation binding to intact plated cells resulted in Kd of 0.48+-0.16 nM and Bmax of 2228+-529 binding sites/cell. In competitive binding experiments, CART(61-102), CART(55-102) and di-iodinated CART(61-102) had similar affinity to PC12 cell membranes, having Ki of 2.99+-0.65, 2.98+-0.33 and 1.27+-0.2 nM, respectively. 125I-CART(61-102) was also bound to intact PC12 cells differentiated using nerve growth factor to the neuronal phenotype.

Byla změřena specifická vazba 125I-CART(61-102) k buňkám PC12 z nádoru krysích nadledvin, intaktním buňkám I membránám. Saturační vazba k celým buňkám byla charakterizována Kd = 0.48+-0.16 nM a Bmax = 2228+-529 vazebných mist na buňku. V kompetitivních vazebných experimentech měl CART(61-102), CART(55-102) a di-jodovaný CART(61-102) podobnou afinitu. 125I-CART(61-102) se take vázal k buňkám PC12 diferencovaným v neurální fenotyp.
Permanent Link: http://hdl.handle.net/11104/0147071