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Volume visualization of biological tissue specimens using confocal microscopy
- 1.0080615 - FGÚ 2007 RIV CZ eng J - Journal Article
Čapek, Martin - Janáček, Jiří - Kubínová, Lucie - Smrčka, P. - Hána, K.
Volume visualization of biological tissue specimens using confocal microscopy.
[Objemová vizualizace biologických tkáňových vzorků s použitím konfokální mikroskopie.]
Lékař a technika. Biomedicinské inženýrství a informatika. Roč. 36, č. 2 (2006), s. 240-244. ISSN 0301-5491.
[Biomedical Engineering Conference of Young Biomedical Engineers and Researchers /2./. Kladno, 19.07.2006-21.07.2006]
R&D Projects: GA MŠMT(CZ) LC06063; GA AV ČR(CZ) IAA100110502; GA AV ČR(CZ) IAA500200510; GA ČR(CZ) GA304/05/0153
Institutional research plan: CEZ:AV0Z50110509
Keywords : 3D reconstruction * confocal microscopy
Subject RIV: JC - Computer Hardware ; Software
We use volume reconstruction as a technique for visualization of biological tissue specimens which are greater than the field of view of a confocal laser scanning microscope. Prior to the volume reconstruction, large specimens are cut into thin physical slices. The first step of volume reconstruction is acquisition of sets of digital volume images (spatial tiles which overlap) from all studied physical slices. The second step is horizontal merging of overlapping spatial tiles of the same physical slice (stitching). The third reconstruction step represents vertical merging of digital volumes of successive physical slices of the specimen. We applied a curve-driven elastic registration algorithm which models deformations by using B-splines. The resulting large digital volumes are visualized using a VolumePro hardware board that offers real-time 3D volume rendering. In this paper we show reconstruction of a laboratory rat oesophagus
Článek popisuje objemovou rekonstrukci a vizualizaci biologických tkáňových vzorku s použitím konfokální mikroskopie, jejichž velikost je větší než zorné pole a hloubka proniku konfokálního mikroskopu. Je využívána registrace obrazů s použitím Euklidovských transformací a elastických deformací modelovaných pomocí B-splinů
Permanent Link: http://hdl.handle.net/11104/0144740
Number of the records: 1