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Screen-printed carbon electrode as a sensor for the detection of DNA hybridization
- 1.0078478 - BFÚ 2007 HR eng A - Abstract
Horáková Brázdilová, Petra - Cahová, Kateřina - Vytřas, K. - Fojta, Miroslav
Screen-printed carbon electrode as a sensor for the detection of DNA hybridization.
[Tištěná uhlíková elektroda jako senzor pro detekci hybridizace DNA.]
Book of Abstracts. Zagreb, 2006. P4-P4.
[13Young Investigators' Seminar on Analytical Chemistry. 05.07.2006-08.07.2006, Zagreb]
R&D Projects: GA MPO(CZ) 1H-PK/42; GA MŠMT(CZ) LC06035; GA AV ČR(CZ) IAA4004402
Institutional research plan: CEZ:AV0Z50040507
Keywords : screen-printed carbon electrode * DNA hybridization * enzyme-linked assay
Subject RIV: BO - Biophysics
We have proposed sensors for enzyme-linked DNA hybridization utilizing screen-printed carbon electrodes (SPCE). Two methods for the detection of DNA hybridization were compared - single-surface and double-surface detection. Oligonucleotides as well as PCR-amplified genomic DNA sequences were utilized as the tDNAs. A good discrimination between the target and nonspecific DNAs was achieved.
Navrhli jsme senzory pro hybridizaci DNA s enzymem značenou sondou využívající uhlíkových tištěných elektrod (SPCE). Byly porovnány dvě metody detekce hybridizace DNA - jednopovrchová a dvoupovrchová. Jako cílové DNA byly detekovány oligonukleotidy a genová DNA amplifikovaná PCR reakcí. Bylo dosaženo dobrého rozlišení mezi cílovou a nespecifickou DNA.
Permanent Link: http://hdl.handle.net/11104/0143581
Number of the records: 1