Effect of microbial colonization on the development of allergic sensitization and mucosal tolerance to birch pollen allergen in mice

0076174 - MBÚ 2007 RIV SIGLE AT eng C - Conference Paper (international conference)
Kozáková, Hana - Repa, A. - Štěpánková, Renata - Hrnčíř, Tomáš - Hudcovic, Tomáš - Hufnagel, K. - Wild, C. - Winkler, B. - Tlaskalová, Helena - Wiedermann, U.
Effect of microbial colonization on the development of allergic sensitization and mucosal tolerance to birch pollen allergen in mice.
[Vliv mikrobiální kolonizace na vývoj alergické senzitizace a slizniční tolerance k pylu břízy u myší.]
Congress of the European Academy of Allergology and Clinical Immunology /25./. Vienna: Basic Science in Allergology, 2006, s. 28-28.
[Congress of the European Academy of Allergology and Clinical Immunology /25./. Vienna (AT), 10.06.2006-14.06.2006]
R&D Projects: GA ČR GA303/05/2249
Institutional research plan: CEZ:AV0Z50200510
Keywords : intestinal microflora * birch pollen allergy
Subject RIV: EE - Microbiology, Virology

The study of the development of mucosal tolerance to major allergic component of birch pollen - Bet v1 in relationship with presence of intestinal microflora was the goal of presented study. Germ-free (GF) or conventionally reared (CV) BALB/c mice were intragastrically or intranasally pre-treated with Bet v 1 prior to sensitization performed by subcutaneous injections of Bet v 1 with aluminum hydroxide as adjuvans. Oral as well as intranasal tolerance induction led to a significant reduction of allergen-specific antibody levels and IgE mediated basophil degranulation, as well as cytokine production in vitro in both GF and CV mice

Vliv střevní mikroflóry na navození slizniční tolerance k pylu břízy byl studován na myším experimentálním modelu. Bezmikrobní a konvenční myši kmene BALB/c byly sondovány Bet v 1 intragastricky a intranasálně a po té byly senzitizovány subkutálně Bet v 1 s hydroxidem sodným. Byla navozena tolerance nezávisle na přítomnosti bakterií, která se odrazila ve snížené hladině IgE a změněné cytokinové produkci
Permanent Link: http://hdl.handle.net/11104/0143344