Organization of extracellular matrix in porcine oocyte- cumulus complexes matured in the presence of proteasomal inhibitor MG132

0053805 - ÚŽFG 2007 RIV FR eng C - Conference Paper (international conference)
Nagyová, Eva - Yi, Y. J. - Procházka, Radek - Šutovsky, M. - Šutovský, P.
Organization of extracellular matrix in porcine oocyte- cumulus complexes matured in the presence of proteasomal inhibitor MG132.
[Extracelulárni matrix” (ECM) v oocyt-kumulárních komplexech (OCC) po kultivaci v mediu s inhibitorem proteazomu (MG 132).]
Final Program and Abstracts from Regulation of Follicle Development and its Clinical Implications. Serono Symposia International Foundation, 2006, s. 1.
[Regulation of Follicle Development and its Clinical Implications. Beaune (FR), 12.05.2006-13.05.2006]
R&D Projects: GA ČR GA305/05/0960
Institutional research plan: CEZ:AV0Z50450515
Keywords : porcine oocyte
Subject RIV: EB - Genetics ; Molecular Biology

MG132 (specific reversible proteasomal inhibitor) inhibited gonadotropin stimulated cumulus expansion of pig OCCs after in vitro culture and decreased significantly synthesis of hyaluronic acid (HA). Covalent transfer of heavy chains from inte-alpha trypsin inhibitor to hyaluronic acid did not occur. At the end of culture period OCCs were compact, since ECM was not organized. These results were also confirmed by immunostaining of ECM –associated components (inte-alpha trypsin inhibitor , tumur-necrosis factor -a-induced protein 6 (TSG-6) and hyaluronic acid .

MG132 (specifický inhibitor proteazomu) inhiboval gonadotropinem stimulovanou expanzi v oocyt-kumularních komplexech prasat po in vitro kultivaci, signifikantně snížil syntézu hyaluronové kyseliny a zabránil tvorbě kovalentní vazby mezi těžkými řetězci inter-alpha trypsin inhibitoru a hyaluronovou kyselinou. Oocyt-kumulární komplexy zůstávaly kompaktni, nedošlo k formováni extracelulární matrix, což bylo potvrzeno také imunodetekcí hlavních komponent ECM (HA, ITI, TSG-6).
Permanent Link: http://hdl.handle.net/11104/0141973