Unique transglycosylation potential of extracellular alpha-D-galactosidase from Talaromyces flavus

0048853 - MBÚ 2007 RIV SIGLE NL eng J - Journal Article
Simerská, Pavla - Kuzma, Marek - Monti, D. - Riva, S. - Macková, M. - Křen, Vladimír
Unique transglycosylation potential of extracellular alpha-D-galactosidase from Talaromyces flavus.
[Jedinečný transglycosylační potenciál extracelulární alpha-D-galactosidasy z Talaromyces flavus.]
Journal of Molecular Catalysis B-Enzymatic. Roč. 39, - (2006), s. 128-134. ISSN 1381-1177
R&D Projects: GA ČR GA203/05/0172; GA MŠMT OC D25.002; GA MŠMT OC D25.001
Institutional research plan: CEZ:AV0Z50200510
Keywords : talaromyces flavus * transglycosylation * tert-butyl alcohol
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.149, year: 2006

The transalycosylation potential of the extracellular alpha-D-galactosidase from the filamentous fungus Talaromyces flavus CCF 2686, chosen as the best enzyme from the screening, was investigated using a series of sterically hindered alcohols (primary, secondary and tertiary) as galactosyl acceptors. Nine alkyl alpha-D-galactopyranosides derived from the following alcohols - tert-butyl alcohol, 2-methyl-2-butyl alcohol, 2-methyl-1-propyl alcohol, 2,2,2-trifluoroethyl alcohol, 2-propyn-1-ol, n-pentyl alcohol, 3,5-dihydroxybenzyl alcohol, 1-phenylethyl alcohol and 1,4-dithio-DL-threitol - were prepared on a semi-preparative scale. This demonstrates a broad synthetic potential of the T flavus alpha-D-galactosidase that has not been observed with another enzyme tested. Moreover, this enzyme exhibits good transglycosylation yields (6-34%). The enzymatic synthesis of tert-butyl alpha-D-galactopyranoside by transglycosylation was studied in detail

Byl studován transglykosylační potenciál extracelulární alpha-D-galactosidasy z Talaromyces flavus s použitím serie stericky bráněných alkoholů
Permanent Link: http://hdl.handle.net/11104/0139382