The effects of reactive oxygen and nitrogen species during yeast replicative ageing

0047844 - MBÚ 2007 RIV SIGLE NL eng J - Journal Article
Wilhelm, J. - Fuksová, H. - Schwippelová, Z. - Vytášek, R. - Pichová, Alena
The effects of reactive oxygen and nitrogen species during yeast replicative ageing.
[Úloha kyslíkových a dusíkových radikálů v průběhu replikativního stárnutí.]
Biofactors. Roč. 27, 1-4 (2006), s. 185-193. ISSN 0951-6433. E-ISSN 1872-8081
R&D Projects: GA ČR(CZ) GA301/03/0289
Institutional research plan: CEZ:AV0Z50200510
Keywords : free radicals * lipofuscin-like pigments * nitrotyrosine
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.095, year: 2006

Free radicals are considered the most important cause of cellular ageing. We have compared the wild type strain with the mutant cells with constitutively active Ras oncogen, which generates increased amounts of free radicals. Increased generation of oxygen-derived free radicals resulted in the Ras mutant cells accumulation of lipofuscin-like pigments during ageing. Ageing wild type cells did not accumulate lipofuscin-like pigments. In contrast, the Ras mutant cells contained decreased levels of alpha tocopherol even in the young cells. This observation indicates that the increased free radical generation can overwhelm the endogenous antioxidant system. We have documented the involvement of nitrogen-derived free radicals in the yeast metabolism

Volné radikály jsou jednou z nejvýznamnějších příčin stárnutí buněk. Byla sledována tvorba volných radikálů kyslíku a dusíku v divokých a mutatních (Ras aktivace) kmenů kvasinek Saccharomyces cerevisiae, dále hladina lipofuscin pigmentu a alfa tokoferolu
Permanent Link: http://hdl.handle.net/11104/0138632