Nucleotides flanking 1,2-d(GpG) adducts formed by enantiomeric analogues of antitumor cisplatin affect termination of DNA synthesis in vitro

0047384 - BFÚ 2007 PT eng A - Abstract
Nováková, Olga - Brabec, Viktor
Nucleotides flanking 1,2-d(GpG) adducts formed by enantiomeric analogues of antitumor cisplatin affect termination of DNA synthesis in vitro.
[Nukleotidy sousedící s 1,2-d(GpG) adukty tvořenými protinádorovými analogy cisplatiny ovlivňují DNA translezovou syntézu in vitro.]
Conference Book, Programme and Abstracts. Aveiro, 2006. s. 159-159. ISBN 989-20-0305-5.
[Eurobic8, 8th European Biological Inorganic Chemistry Conference and Eurobic Young Researchers Forum. 01.07.2006-06.07.2006, Aveiro]
R&D Projects: GA ČR(CZ) GA203/06/1239
Institutional research plan: CEZ:AV0Z50040507
Keywords : termination of DNA synthesis * Klenow fragment * platinum drugs
Subject RIV: BO - Biophysics

To study the mechanism underlying termination of DNA synthesis on platinated templates we tested the efficiency of Klenow fragment of DNA polymeraseI, to catalyze primer elongation across 1,2-d(GpG) adducts formed by enantiomeric [PtCl2(DAB)] and [PtCl2(DACH)] compounds. We have found that differences in the ability of Klenow fragment to bypass 1,2-d(GpG) adducts formed by enantiomeric [PtCl2(DAB)] and [PtCl2(DACH)] compounds depend on the ligand size or type and mainly the bases flanking the adduct.

Byl studován mechanizmus polymerázové reakce na syntetických oligonukleotidech modifikovaných protinádorově aktivními analogy cisplatiny- [PtCl2(DAB)] a [Ptcl2(DACH)]. Ukázali jsme, že katalytická aktivita Klenowova fragmentu DNA polymerázy I byla závislá jak na struktuře a orientaci ligandů vybraných komplexů, tak na sekvenci nukleotidů sousedících s modifikovanými guaniny.
Permanent Link: http://hdl.handle.net/11104/0138310