Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research

0043203 - ÚFCH JH 2007 RIV US eng J - Journal Article
Benda, Aleš - Fagulová, Veronika - Deyneka, Alexander - Enderlain, J. - Hof, Martin
Fluorescence lifetime correlation spectroscopy combined with lifetime tuning: New perspectives in supported phospholipid bilayer research.
[Fluorescenční korelační spektroskopie: Nové perspektivy ve výzkumu fosfolipidových vrstev.]
Langmuir. Roč. 22, č. 23 (2006), s. 9580-9585. ISSN 0743-7463
R&D Projects: GA ČR GA203/05/2308; GA MŠMT LC06063
Institutional research plan: CEZ:AV0Z40400503; CEZ:AV0Z10100522
Keywords : spectroscopy * fluorescence * FLCS
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 3.902, year: 2006

A new concept based on fluorescence lifetime correlation spectroscopy (FLCS) is presented allowing the simultaneous determination of diffusion coefficients of identical molecules located in different environments. The difference in fluorescence lifetimes, which is the main prerequisite for FLCS, is reached by locating one polution of the dye close to a light-absorbing surface. Since such surfaces quench fluorescence, the fluorescence lifetime of chromophores located close to these surfaces can be tuned in a specific manner. This approach has been demonstrated for a BODIPY-tail-labeled lipid in supported phospholipid bilayers (SPBs) as well as in phospholipid multilayers adsorbed onto solid supports. In particular, the effect of the solid support type on the fluorescence lifetime as well as its dependence on the BODIPY-support distance has been characterized and verified by theoretical considerations based on precise determination of refractive indices of the supports.

V této práce se zaměřujeme na nové koncepty výzkumu fluorescenční korelační spektroskopie (FLCS) a na nové perspektivy ve výzkumu fosfolipidových vrstev.
Permanent Link: http://hdl.handle.net/11104/0003682