Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy

0043199 - ÚFCH JH 2007 RIV US eng J - Journal Article
Humpolíčková, Jana - Gielen, E. - Benda, Aleš - Fagulová, Veronika - Vercammen, J. - van de Ven, M. - Hof, Martin - Ameloot, M. - Engelborghs, Y.
Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy.
[Výzkum difúzních zákonitostí buněčných membrán pomocí fluorescenční korelační spektroskopie.]
Biophysical Journal. Roč. 91, č. 3 (2006), L23-L25. ISSN 0006-3495. E-ISSN 1542-0086
R&D Projects: GA ČR GA203/05/2308; GA MŠMT LC06063
Grant - others:Research Council of the UHasselt and tUL(BE) GOA/2006/02; Research Council of the UHasselt and tUL(BE) BIL03/08
Institutional research plan: CEZ:AV0Z40400503
Keywords : model membranes * rafts * organization
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 4.757, year: 2006

The plasma membrane of various mammalian cell types is heterogeneous in structure and may contain microdomains, which can impose constraints on the lateral diffusion of its constituents. Fluorescence correlation spectroscopy (FCS) can be used to investigate the dynamic properties of the plasma membrane of living cells. Very recently, Wawrezinieck et al. (Wawrezinieck, L., H. Rigneault, D. Marguet, and P. F. Lenne. 2005. Biophys. J. 89:4029-4042) described a method to probe the nature of the lateral microheterogeneities of the membrane by varying the beam size in the FCS instrument. The dependence of the width of the autocorrelation function at half-maximum, i.e., the diffusion time, on the transverse area of the confocal volume gives information on the nature of the imposed confinement.

Tato práce pojednává a výzkumu plasmových membrán, jejich typů a rozdílů ve struktuře pomocí fluorescenční korelační spektroskopie (FCS).
Permanent Link: http://hdl.handle.net/11104/0136253