Role of the carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues

0043116 - ÚMG 2007 RIV GR eng J - Journal Article
Marková, Vladimíra - Smetana Jr, K. - Jeníková, Gabriela - Láchová, Jitka - Krejčiříková, Veronika - Poplštein, M. - Fábry, Milan - Brynda, Jiří - Alvarez, R. - Cummings, R. - Malý, Petr
Role of the carbohydrate recognition domains of mouse galectin-4 in oligosaccharide binding and epitope recognition and expression of galectin-4 and galectin-6 in mouse cells and tissues.
[Úloha uhlovodíkových rekogničních domén myšího galektinu-4 ve vazbě oligosacharidů a rozpoznávání epitopů a exprese galektinu-4 a galektinu-6 v myších buňkách a tkáních.]
International Journal of Molecular Medicine. Roč. 18, č. 1 (2006), s. 65-76. ISSN 1107-3756. E-ISSN 1791-244X
R&D Projects: GA ČR(CZ) GA304/03/0090
Grant - others:National Institute of General Medical Sciences(US) GM62116
Institutional research plan: CEZ:AV0Z50520514
Keywords : galectin * glycoprotein binding * lectin-carbohydrate
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.854, year: 2006

Galectin-4 and galectin-6 found in the mouse are closely related tandem-repeat members of the galectin family. We studied the expression of both galectins in mouse tissues, cell lines and early mouse embryogenesis. To study the role of carbohydrate recognition domains (CRDs) in oligosaccharide binding and epitope recognition, we cloned mouse full-length galectin-4 and galectin-6 cDNA and constructed bacterial expression vectors producing histidine-tagged recombinant galectin-4 and its truncated CRD1 and CRD2 forms. Preparation of these recombinant forms of mouse galectin-4 and its CRD domains allowed us to investigate the oligosaccharide binding profile using Glycan Array analysis of 185 different glycan structures.

Studovali jsme expresi galektinu-4 a galektinu-6 v myších tkáních a bunečných liniích. Připravili jsme rekombinantní CRD domény galektinu-4 a galektinu-6 s histidinovou kotvou a dále studovali vazbu oligosacharidů na CRD domény galektinu-4 pomocí Glycan array analýzy.
Permanent Link: http://hdl.handle.net/11104/0136199