Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats

0039333 - MBÚ 2007 RIV SIGLE NL eng J - Journal Article
Kubáč, David - Čejková, A. - Masák, J. - Jirků, V. - Lemaire, M. - Gallienne, E. - Bolte, J. - Stloukal, R. - Martínková, Ludmila
Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats.
[Biotransformace nitrilů pomocí baktérie Rhodococcus equi A4 imobilizované v LentiKats.]
Journal of Molecular Catalysis B-Enzymatic. Roč. 39, - (2006), s. 59-61. ISSN 1381-1177
R&D Projects: GA ČR GA203/05/2267; GA MŠMT OC D25.001; GA AV ČR IAA4020213
Institutional research plan: CEZ:AV0Z50200510
Keywords : nitrile hydratase * amidase * nitriles
Subject RIV: EE - Microbiology, Virology
Impact factor: 2.149, year: 2006

Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles. LentiKats (R). The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylenebutanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenylpropanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was most stable during the transformation of (R,S)-3-hydroxy-2-methylenebutanenitrile. No significant loss of the amidase activity was observed within the course of the biocatalytic reaction

Celé buňky baktérie Rhodococcus equi A4, producenta nitrilhydratasy a amidasy, byly imobilizovány v čočkovitých částicích hydrogelu, LentiKats (R). Imobilizovaný biokatalyzátor byl použit pro biotransformaci benzonitrilu, 3-kyanopyridinu, (R,S)-3-hydroxy-2-methylenebutanenitrilu and (R,S)-3-hydroxy-2-methylene-3-phenylpropanenitrilu. Stabilita nitrilhydratasy během opakovaného použití biokatalyzátoru závisela na typu substrátu. Enzym byl stabilní během transformace (R,S)-3-hydroxy-2-methylenebutanenitrilu. Během reakce nebyla pozorována výraznější ztráta aktivity amidasy
Permanent Link: http://hdl.handle.net/11104/0133451