Cloning and molecular analysis of the regulatory factor HiMyb1 in hop (Humulus lupulus L.) and the potential of hop to produce bioactive prenylated flavonoids

0027852 - UMBR-M 2006 RIV US eng J - Journal Article
Matoušek, Jaroslav - Vrba, Lukáš - Novák, Petr - Patzak, J. - De Keukeleire, J. - Škopek, Josef - Heyerick, A. - Roldán-Ruiz, I. - De Keukeleire, D.
Cloning and molecular analysis of the regulatory factor HiMyb1 in hop (Humulus lupulus L.) and the potential of hop to produce bioactive prenylated flavonoids.
[Klonování a molekulární analýza regulačního faktoru HlMyb1 chmelu (Humulus lupulus L.) a potenciál chmelu produkovat bioaktivní prenylované flavonoidy.]
Journal of Agricultural and Food Chemistry. Roč. 53, - (2005), s. 4793-4798. ISSN 0021-8561. E-ISSN 1520-5118
R&D Projects: GA ČR(CZ) GA521/03/0072
Institutional research plan: CEZ:AV0Z50510513
Keywords : molecular analysis * Humulus lupulus L.
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.507, year: 2005

The cDNA of the first Myb regulatory factor from hop, called HIMyb1, was cloned and analyzed. The HIMyb1 open reading frame encodes 272 amino acids (29.8 kDa), and the protein showed highest homology to the light-regulated factor AtMyb68 from Arabidopsis thaliana within the Myb domain, whereas there was no significant homology with known MYB proteins outside this domain.

Byla klonována a analyzována cDNA prvního regulačního faktoru Myb z chmele nazvaná HlMyb1. ORF HlMyb1 kóduje 272 aminokyselin (29.8 kDa) a protein vykazuje nejvyšší homologii k světlem regulovanému faktoru AtMyb68, patřícímu k doméně Myb z Arabidopsis thaliana, zatímco se známými proteiny mimo tuto doménu nebyla zjištěna žádná homologie.
Permanent Link: http://hdl.handle.net/11104/0117887