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Efficient compensation of the light attenuation with depth in confocal microscopy

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    0027288 - FGÚ 2006 RIV CZ eng K - Conference Paper (Czech conference)
    Čapek, Martin - Kubínová, Lucie
    Efficient compensation of the light attenuation with depth in confocal microscopy.
    [Účinná kompenzace útlumu jasu a kontrastu s hloubkou v konfokální mikroskopii.]
    Proceedings of WORKSHOP 2005. Praha: ČVUT Praha, 2005, s. 772-773. ISBN 80-01-03201-9.
    [WORKSHOP 2005. Czech Technical University in Prague /14./. Praha (CZ), 21.03.2005-25.03.2005]
    R&D Projects: GA AV ČR(CZ) KJB6011309; GA MŠMT(CZ) ME 688
    Grant - others:CZ-SK(CZ) KONTAKT 139; CZ-GR(CZ) KONTAKT RC_3_19
    Institutional research plan: CEZ:AV0Z5011922
    Keywords : confocal microscopy * light attenuation
    Subject RIV: JD - Computer Applications, Robotics

    Images captured by a confocal laser scanning microscope from deep layers of the specimen are often darker than images from the top most layers due to absorption and scattering of both excitation and fluorescent light. These effects cause problems in subsequent analysis of biological objects. Therefore, methods for compensation of the light attenuation with depth must be used

    Pokles jasu a kontrastu s hloubkou zaostření v konfokální mikroskopii se dá kompenzovat jak optimalizací mikroskopu během snímání, tak pomocí vhodných algoritmů zpracování dat po nasnímání
    Permanent Link: http://hdl.handle.net/11104/0117403

     
     
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