Characterization of polymers by using new sample deposition device developed for off-line combination of supercritical fluid chromatography and MALDI-TOF mass spectrometry

0023643 - ÚIACH 2006 IT eng C - Conference Paper (international conference)
Chmelík, Josef - Řehulka, Pavel - Planeta, Josef
Characterization of polymers by using new sample deposition device developed for off-line combination of supercritical fluid chromatography and MALDI-TOF mass spectrometry.
20th Informal Meeting on Mass Spectrometry. Padova: Servizi Grafici Editoriali, 2002 - (Traldi, P.; Seraglia, R.; Marotta, E.), s. 35. ISBN 88-86281-69-2.
[Informal Meeting on Mass Spectrometry /20./. Fiera di Primiero (IT), 12.05.2002-16.05.2002]
R&D Projects: GA AV ČR IAA4031104
Institutional research plan: CEZ:AV0Z40310501
Keywords : mass spectrometry MALDI-TOF * supercritical fluid chromatography * off-line connection
Subject RIV: CB - Analytical Chemistry, Separation

Molecular mass distributions of silicone oil samples were determined by supercritical fluid chromatography (SFC), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOF MS) and their off-line combination. SFC with micropacked capillary columns was used to separate a silicone oil sample into several fractions. These fractions were sprayed from an SFC chromatographic column directly on a MALDI target by using the developed sample deposition device. The sample deposition device is based on a common plotter unit, complemented by a microcontroller PICI6C84. The unit is connected by an RS-232 interface to a PC with the main control software running under MS Windows. The new sample deposition device made the off-line combination SFC-MALDI simpler, faster and more sensitive. Based on MALDI- TOF analysis of the fractions polymer size distribution of the sample was calculated
Permanent Link: http://hdl.handle.net/11104/0112254