Coaxial Nanofibrous Scaffold Prepared Using Centrifugal Spinning as a Drug Delivery System for Skeletal Tissue Engineering

Article Preview

Abstract:

Skeletal disorders, caused by trauma, disease, or carcinoma, may result in tissue loss and, finally, in endoprosthesis. Tissue engineering offers an alternative - tissue scaffolds. Its constructs may be seeded with autologous cells or, alternatively, attract cells from the surrounding tissues. Such a scaffold must meet several requirements, such as biocompatibility, biodegradability and suitable morphology for cell attachment and proliferation. Nonetheless, scaffold should stimulate cells migrated from the surrounding tissues to infiltrate the scaffold, proliferate and differentiate to the required cell type. In the current study, we developed a fibrous scaffold with 3D structure using emulsion centrifugal spinning. The scaffold from poly-ɛ-caprolactone contained a cocktail of growth factors, i.e. TGF-β, IGF and bFGF. The released growth factors enhanced cell proliferation and chondrogenic differentiation. The scaffold is a promising material for skeletal tissue engineering.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

162-168

Citation:

Online since:

March 2020

Export:

Price:

* - Corresponding Author

[1] M. Rampichová, M. Buzgo, J. Chvojka, E. Prosecká, O. Kofronová, and E. Amler, Cell penetration to nanofibrous scaffolds: Forcespinning®, an alternative approach for fabricating 3D nanofibers,, Cell Adhesion and Migration, vol. 8, no. 1. p.36–41, (2014).

DOI: 10.4161/cam.27477

Google Scholar

[2] M. Rampichová et al., Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors,, Int. J. Nanomedicine, vol. 12, p.347–361, (2017).

DOI: 10.2147/ijn.s120206

Google Scholar

[3] M. Rampichová, M. Buzgo, J. Chvojka, E. Prosecká, O. Kofronová, and E. Amler, Cell penetration to nanofibrous scaffolds: Forcespinning®, an alternative approach for fabricating 3D nanofibers,, Cell Adhes. Migr., vol. 8, no. 1, (2014).

DOI: 10.4161/cam.27477

Google Scholar

[4] K. Vocetkova et al., A comparison of high throughput core-shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells,, RSC Adv., (2017).

DOI: 10.1039/c7ra08728d

Google Scholar

[5] M. Buzgo et al., Emulsion centrifugal spinning for production of 3D drug releasing nanofibres with core/shell structure,, RSC Adv., vol. 7, no. 3, (2017).

DOI: 10.1039/c6ra26606a

Google Scholar

[6] T. A.G., F. J., S. R., P. G., H. M., and M. I., Cell yield, proliferation, and postexpansion differentiation capacity of human ear, nasal, and rib chondrocytes,, Tissue Eng., (2004).

DOI: 10.1089/1076327041348572

Google Scholar

[7] C. Phornphutkul, K. Y. Wu, X. Yang, Q. Chen, and P. A. Gruppuso, Insulin-like growth factor-I signaling is modified during chondrocyte differentiation,, J. Endocrinol., (2004).

DOI: 10.1677/joe.1.05873

Google Scholar

[8] S. Miot et al., Cartilage tissue engineering by expanded goat articular chondrocytes,, J. Orthop. Res., (2006).

Google Scholar

[9] M. Rampichová et al., Platelet-functionalized three-dimensional poly-ε-caprolactone fibrous scaffold prepared using centrifugal spinning for delivery of growth factors,, Int. J. Nanomedicine, vol. 12, (2017).

DOI: 10.2147/ijn.s120206

Google Scholar

[10] S. M. Seyedin, D. M. Rosen, and P. R. Segarini, Modulation of chondroblast phenotype by transforming growth factor-beta.,, Pathol. Immunopathol. Res., vol. 7, no. 1–2, p.38–42, (1988).

DOI: 10.1159/000157090

Google Scholar

[11] R. Tuli et al., Transforming Growth Factor-β-mediated Chondrogenesis of Human Mesenchymal Progenitor Cells Involves N-cadherin and Mitogen-activated Protein Kinase and Wnt Signaling Cross-talk,, J. Biol. Chem., (2003).

DOI: 10.1074/jbc.m305312200

Google Scholar

[12] S. E. Kim et al., Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β1: Implications for cartilage tissue engineering,, J. Control. Release, (2003).

DOI: 10.1016/s0168-3659(03)00274-8

Google Scholar

[13] E. Filová et al., Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee,, J. Biomed. Mater. Res. - Part B Appl. Biomater., (2008).

DOI: 10.1002/jbm.b.31119

Google Scholar

[14] M. Mastrogiacomo, R. Cancedda, and R. Quarto, Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells,, Osteoarthr. Cartil., (2001).

DOI: 10.1053/joca.2001.0442

Google Scholar

[15] R. S. Nirmal and P. D. Nair, Significance of soluble growth factors in the chondrogenic response of human umbilical cord matrix stem cells in a porous three dimensional scaffold,, Eur. Cells Mater., (2013).

DOI: 10.22203/ecm.v026a17

Google Scholar