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Volume 72, Issue 8

gen. nov., sp. nov., a bacterium isolated from soil with the aid of culture supernatant containing resuscitation-promoting factor No Access

Abstract

An orange-golden iridescent culture, designated A1X5R2, was isolated from a compost soil suspension which was amended with NCTC 2665 culture supernatant. The cells were non-motile, Gram-stain-negative, 0.4–0.5 µm wide and 0.7–1.4 µm long. The 16S rRNA-based phylogenetic and whole-genome analyses revealed that strain A1X5R2 forms a distinct lineage within the family and is closely related to members of the genus (, 93.04 % similarity, and , 92.77 %). The organism grew at 22–47 °C (optimal at 37 °C), salinity <3 % (optimal at 1.5 %) and at pH 7. The major respiratory quinone was ubiquinone-10, but a small quantity of ubiquinone-9 was also detected The major polyamine was homospermidine, but a small quantity of putrescine was also detected. The strain contained Cω7, C, C ω7 and C as the major fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, sphingoglycolipid, diphosphatidylglycerol, two unidentified phospholipids and three unidentified amino lipids. The DNA G+C content was 64.9 mol%. According to the results of phylogenetic and phylogenomic analyses, as well as its physiological characteristics, strain A2X5R2 represents the type species of a novel genus within the family . The name gen. nov., sp. nov. is proposed, with the type strain being A1X5R2 (=NCCB 100839=DSM 112829).

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  • Accepted:
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Keyword(s): compost soil , extracellular organic matter , resuscitation-promoting factor , soil bacteria , Sphingosinicellaceae and whole genome average nucleotide identity
© 2022 The Authors
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2022-08-05
2024-04-24
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Pedomonas mirosovicensis gen. nov., sp. nov., a bacterium isolated from soil with the aid of Micrococcus luteus culture supernatant containing resuscitation-promoting factor
Int J Syst Evol Microbiol 72, 005467 (2022); https://doi.org/10.1099/ijsem.0.005467
/content/journal/ijsem/10.1099/ijsem.0.005467
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