Hort. Sci. (Prague), 2022, 49(3):179-188 | DOI: 10.17221/19/2021-HORTSCI

Content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes - the influence of samples dryingOriginal Paper

Naděžda Vrchotová1, Jan Tříska ORCID...*,1, Radomíra Střalková2, Štěpán Horník3, Jan Sýkora3, Josef Balík4, Ivo Soural4, Lukáš Toupal5, Radek Sotolář4
1 Laboratory of Metabolomics and Isotopic Analyses, Global Change Research Institute, Brno, Czech Republic
2 Research Station for Viticulture Karlštejn, Crop Research Institute, Prague, Czech Republic
3 Department of Analytical Chemistry, Institute of Chemical Process Fundamentals, Prague, Czech Republic
4 Faculty of Horticulture, Mendel University in Brno, Lednice, Czech Republic
5 Department of Chemistry, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic

The main objective was to study the effect of sample preparation on the content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes. The samples of six varieties of Vitis vinifera L. ('Hibernal', 'Malverina', 'Kolor', 'Fioletovij Augustovskij', 'Grüner Veltliner' and 'Blaufränkisch') were either lyophilised or slow dried at room temperature (three months), extracted and analysed by HPLC using DAD and FLD detectors and by LC-MS. The presence of trans-δ-viniferin was confirmed by comparison with trans-δ-viniferin prepared by dimerization of trans-resveratrol using laccase and its structure was verified by LC-NMR. The slow drying of the samples at room temperature enables the synthesis of a significant amount of stilbenes in all the studied varieties of V. vinifera canes resulting in a higher stilbene content compared to the lyophilised samples. The results of this study show the importance of the method of drying the cane sample before the extraction and analysis.

Keywords: viniferins; Vitis vinifera; grapevine cane; lyophilization; room temperature drying; LC-MS

Published: September 30, 2022  Show citation

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Vrchotová N, Tříska J, Střalková R, Horník Š, Sýkora J, Balík J, et al.. Content of trans-resveratrol, trans-ε-viniferin and trans-δ-viniferin in young spring grapevine canes - the influence of samples drying. Hort. Sci. (Prague). 2022;49(3):179-188. doi: 10.17221/19/2021-HORTSCI.
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