Photosynthetica 2023, 61(4):483-491 | DOI: 10.32615/ps.2023.043

Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy

R. KAŇA1, B. ŠEDIVÁ1, 2, O. PRÁŠIL1
1 Centre Algatech, Institute of Microbiology of the Czech Academy of Sciences, Opatovický mlýn, 379 81 Třeboň, Czech Republic
2 Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 31a, 370 05 České Budějovice, Czech Republic

The investigation of spatial heterogeneity within the thylakoid membrane (TM) proteins has gained increasing attention in photosynthetic research. The recent advances in live-cell imaging have allowed the identification of heterogeneous organisation of photosystems in small cyanobacterial cells. These sub-micrometre TM regions, termed microdomains in cyanobacteria, exhibit functional similarities with granal (Photosystem II dominant) and stromal (Photosystem I dominant) regions observed in TM of higher plants. This study delves into microdomain heterogeneity using super-resolution Airyscan-based microscopy enhancing resolution to approximately ~125 nm in x-y dimension. The new data reveal membrane areas rich in Photosystem I within the inner TM rings. Moreover, we identified analogous dynamics in the mobility of Photosystem II and phycobilisomes; countering earlier models that postulated differing mobility of these complexes. These novel findings thus hold significance for our understanding of photosynthesis regulation, particularly during state transitions.

Additional key words: Airyscan; cyanobacteria; FRAP; microdomain; photosystem; protein mobility; super-resolution microscopy; thylakoid membrane heterogeneity.

Received: November 3, 2023; Revised: November 27, 2023; Accepted: December 1, 2023; Prepublished online: December 18, 2023; Published: December 19, 2023  Show citation

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KAŇA, R., ŠEDIVÁ, B., & PRÁŠIL, O. (2023). Microdomains heterogeneity in the thylakoid membrane proteins visualized by super-resolution microscopy. Photosynthetica, 61(SPECIAL ISSUE 2023-2), 483-491. doi: 10.32615/ps.2023.043
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