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Plant Cell Morphogenesis pp 63–76Cite as

Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

Abstract

In this chapter, conventional techniques are described for the preparation of plant samples at room temperature before examination in the high resolution scanning electron microscopy. Protocols are given on how to collect, to fix, to dehydrate, and to dry plant samples. Subsequently, it is described how to stick them to stubs and cover with a thin conductive layer. These methods are suitable for a wide variety of plant specimens, ranging from microalgae to higher plants.

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Acknowledgments

I acknowledge the Czech-BioImaging large RI project (LM2015062 funded by MEYS CR) and by European Regional Development Fund-Project “National infrastructure for biological and medical imaging” (No. CZ.02.1.01/0.0/0.0/16_013/0001775) for their support.

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Authors and Affiliations

  1. Biology Centre of CAS, Institute of Parasitology, České Budějovice, Czech Republic

    Jana Nebesářová

  2. Faculty of Science, Charles University, Prague, Czech Republic

    Jana Nebesářová

Authors
  1. Jana Nebesářová
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Correspondence to Jana Nebesářová .

Editor information

Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Nebesářová, J. (2019). Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

  • eBook Packages: Springer Protocols

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