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Structural Analysis of Strigolactone-Related Gene Products

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Strigolactones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2309))

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Abstract

Structural knowledge of biological macromolecules is essential for understanding their function and for modifying that function by engineering. Protein crystallography is a powerful method for elucidating molecular structures of proteins, but it is essential that the investigator has a basic knowledge of good practices and of the major pitfalls in the technique. Here we describe issues specific for the case of structural studies of strigolactone (SL) receptor structure and function, and in particular the difficulties associated with capturing complexes of SL receptors with the SL hormone ligand in the crystal.

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Acknowledgments

This work was supported by a COST grant from the European Union (STREAM Action no. FA1206). Part of this work was also supported by the project “Structural dynamics of biomolecular systems (ELIBIO)” (NO. CZ.02.1.01/0.0/0.0/15_003/0000447) from the European Regional Development Fund.

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Authors and Affiliations

  1. Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden

    Inger Andersson, Gunilla H. Carlsson & Dirk Hasse

  2. Arctic University of Norway, Tromsø, Norway

    Inger Andersson

  3. Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic

    Inger Andersson

Authors
  1. Inger Andersson
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  2. Gunilla H. Carlsson
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  3. Dirk Hasse
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Corresponding author

Correspondence to Inger Andersson .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Turin, Turin, Italy

    Cristina Prandi

  2. DISAFA-PlantStressLab, University of Turin, Grugliasco, Italy

    Francesca Cardinale

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Andersson, I., Carlsson, G.H., Hasse, D. (2021). Structural Analysis of Strigolactone-Related Gene Products. In: Prandi, C., Cardinale, F. (eds) Strigolactones. Methods in Molecular Biology, vol 2309. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1429-7_19

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  • DOI: https://doi.org/10.1007/978-1-0716-1429-7_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1428-0

  • Online ISBN: 978-1-0716-1429-7

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