Abstract
Monoxenous (one host) trypanosomatids from insects and other invertebrates can be introduced into axenic culture relatively easily and efficiently, allowing for their transfer from the field into the laboratory. Here we describe simple methods and alternative cultivation protocols, the wider application of which will allow substantial expansion of trypanosomatids available for research.
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Acknowledgments
Support from the Czech Grant Agency 16-18699S, ERC CZ LL1601 and the ERD Funds (project OPVVV 16_019/0000759) is acknowledged.
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Lukeš, J., Votýpka, J. (2020). Field Isolation and Cultivation of Trypanosomatids from Insects. In: Michels, P., Ginger, M., Zilberstein, D. (eds) Trypanosomatids. Methods in Molecular Biology, vol 2116. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0294-2_1
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DOI: https://doi.org/10.1007/978-1-0716-0294-2_1
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