Abstract
Leafhoppers (Cicadellidae) occur in all biogeographical regions and feed on a wide range of host plants. Classic identification of leafhoppers is based only on the morphology of male genitalia, while DNA barcoding is an effective tool to identify different leafhopper species in any stage of their life cycle. Phylogenetic relationships among leafhoppers remain unexplored, but recent phylogenies have begun to interpret the evolutionary relationships of previously recognized tribes and subfamilies. Here we used mitochondrial Cytochrome Oxidase I sequences to barcode and determine the phylogenetic relationship among eight leafhopper genera collected from Egypt. Eight leafhopper samples from eight different genera were collected from various plants using a sweeping net and aspirator. Males of seven species were available while females only were available from the one genus (Austroagallia sp.). Novel leafhopper-specific mtDNA COI degenerate primers were designed. Our results showed that the mean of intraspecific COI sequence divergence in leafhoppers was 0.45%, with a range from 0.15% to 1.23%, and the divergences of most species were over 2%. For the first time, we were exploring the evolutionary relationships among surveyed genera (Austroagallia, Empoasca, Eupteryx, Exitianus, Macrosteles, Nephotettix, and Orosius) using a robust phylogenetic supertree. Our results demonstrate that DNA barcoding is likely to be a powerful tool for identifying and understanding leafhoppers’ evolution.
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Acknowledgments
Authors would like to express deep thank for Prof. Dr. Christopher H. Dietrich of the Illinois Natural History Survey, USA for his invaluable help for identification Austroagallia sp. according to female morphological characters. The authors would like to thank ERDF/ESF Centre for research of pathogenicity, and virulence of parasites grant number [No. CZ.02.1.01/0.0/0.0/16_019/0000759] for funding the sequencing. Computational resources were provided by the ELIXIR-CZ project (LM2015047), part of the international ELIXIR infrastructure.
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Emam, A.K., Ibrahim, H.E., Helmi, A. et al. Identification of some Egyptian leafhopper species (Hemiptera: Cicadellidae) using DNA barcoding. Biologia 75, 1337–1346 (2020). https://doi.org/10.2478/s11756-019-00384-y
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DOI: https://doi.org/10.2478/s11756-019-00384-y