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Description of Raphidascaris (Ichthyascaris) multipapillata sp. nov., Redescription of Hysterothylacium simile Li et al., 2013 and New Records of Some Other Ascaridoid and Seuratoid Nematodes from Marine Fishes in Japan

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Abstract

Purpose

Many nematode species parasitizing marine fishes were reported from Japanese waters. However, considering the wealth of fish species in this zoogeographically interesting region and the host specificity of nematodes, the number of hitherto recorded fish nematodes probably represents only a very small part of the potential Japanese fauna of these organisms. Therefore, new data are needed to recognise the species composition of these parasites in this region.

Methods

Helminthological examinations of some marine fishes from off Japan, carried out in the years 2005–2017, revealed one new and five insufficiently known species of ascaridoid and seuratoid nematodes. These were studied with the use of light and scanning electron microscopy.

Results

All six nematode species recorded are described: Anisakidae: Raphidascaris (Ichthyascaris) multipapillata sp. nov. (male and females) from the intestine of Labracoglossa argenteiventris Peters (Kyphosidae) in the Sea of Japan, Raphidascaris (Ichthyascaris) sp. (third-stage larvae) probably from the digestive tract of Seriola dumerili (Risso) and S. rivoliana Valenciennes (both Carangidae) in the western North Pacific Ocean off Ishigaki-jima Island, Okinawa Prefecture, Hysterothylacium simile Li et al., 2013 (males and females) from the stomach of Lateolabrax japonicus (Cuvier) (Lateolabracidae) in the Sea of Japan, Hysterothylacium sp. 1 (female) from the pyloric caeca of Jaydia lineata (Temminck et Schlegel) (Apogonidae) in the Seto Inland Sea, Hysterothylacium sp. 2 (third- and fourth-stage larvae) from the intestine and mesentery of Etelis coruscans Valenciennes (Lutjanidae) and Pseudorhombus pentophthalmus Günther (Paralichthyidae) in the western North Pacific Ocean off Ishigaki-jima Island, Okinawa Prefecture and the Seto Inland Sea, respectively; and Cucullanidae: Cucullanus sp. (female) from the intestine of Semicossyphus reticulatus (Valenciennes) (Labridae) in the Seto Inland Sea. Raphidascaris (I.) multipapillata sp. n. differs from congeners mainly in the high number (79) of pairs of caudal papillae, spicules 402 µm long and in the structure of the male and female tail tips. Hysterothylacium simile is redescribed, being recorded from off Japan for the first time.

Conclusions

In addition to the discovery of the new nematode species R. (I.) multipapillata sp. nov. and a redescription of H. simile providing some new morphological data of this parasite, the findings of all other species represent new host and geographical records. The present study extends the knowledge of the species composition and host–parasite relationships of nematodes parasitizing marine fishes.

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Acknowledgements

We thank Masaya Iida (Japan Sea National Fisheries Research Institute) and Yuichiro Ochi (Hiroshima University) for providing nematode samples from L. japonicus and S. reticulatus, respectively. We are grateful to the staff of the Research Center for Subtropical Fisheries (Seikai National Fisheries Research Institute, Fisheries Research Agency) for providing laboratory facilities. We acknowledge Kohei Ito (Hiroshima University) and Hirotaka Katahira (Azabu University) for assistance with fish sampling. Thanks are also due to the Laboratory of Electron Microscopy, Institute of Parasitology, Biology Centre CAS, institution supported by the MEYS CR (LM2015062 Czech-BioImaging) for their support with obtaining scientific data presented in this paper, and to Blanka Škoríková of the same Institute for help with the illustrations.

Funding

This study was partly supported by the institutional support of the Institute of Parasitology, BC AS CR (RVO: 60077344).

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Authors and Affiliations

  1. Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic

    František Moravec

  2. Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8528, Japan

    Kazuya Nagasawa

  3. Graduate School of Science, Kobe University, Nada, Kobe, Hyogo, 657-8501, Japan

    Masato Nitta

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  1. František Moravec
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Correspondence to František Moravec.

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Moravec, F., Nagasawa, K. & Nitta, M. Description of Raphidascaris (Ichthyascaris) multipapillata sp. nov., Redescription of Hysterothylacium simile Li et al., 2013 and New Records of Some Other Ascaridoid and Seuratoid Nematodes from Marine Fishes in Japan. Acta Parasit. 66, 461–474 (2021). https://doi.org/10.1007/s11686-020-00299-8

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