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Complete genome sequence of Xanthomonas phage M29, a new member of Foxunavirus isolated in the Czech Republic

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Abstract

The newly discovered Xanthomonas phage M29 (Xp M29) is the first lytic phage infecting Xanthomonas campestris pv. campestris (Xcc) that was isolated from cabbage leaves in the Czech Republic. The phage consists of icosahedral head approximately 60 nm in diameter and a probably contractile tail of 170 nm. The complete genome size was 42 891 bp, with a G + C content of 59.6%, and 69 ORFs were predicted on both strands. Pairwise nucleotide comparison showed the highest similarity with the recently described Xanthomonas phage FoX3 (91.2%). Bacteriophage Xp M29 has a narrow host range infecting 5 out of 21 isolates of Xcc. Xp M29 is a novel species in a newly formed genus Foxunavirus assigned directly to the class Caudoviricetes.

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Fig.1
Fig. 2

Data availability

The sequence is available in GenBank under the mentioned accession number.

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Acknowledgements

We are grateful to Simona Buchtova for her help with the laboratory work.

Funding

This work was financed with the state support of the Technology Agency of the Czech Republic within the Gama 2 program and with the institutional support RVO60077344 of the Czech Academy of Sciences and cofinanced with the Internal Grant Agency of MENDELU (IGA-ZF/2021-SI1002).

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Authors and Affiliations

  1. Faculty of Horticulture, Mendeleum-Institute of Genetics, Mendel University in Brno, Valtická 334, 691 44, Brno, Lednice, Czech Republic

    Mária Neoralová & Aleš Eichmeier

  2. Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, 370 05, České Budějovice, Czech Republic

    Sára Brázdová & Karel Petrzik

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  1. Mária Neoralová
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  2. Sára Brázdová
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  3. Aleš Eichmeier
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  4. Karel Petrzik
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Contributions

All authors contributed to the study conception and design. Material preparation, data processing and analysis were performed by MN and SB. The first draft of the manuscript was written by MN and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Mária Neoralová.

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Neoralová, M., Brázdová, S., Eichmeier, A. et al. Complete genome sequence of Xanthomonas phage M29, a new member of Foxunavirus isolated in the Czech Republic. Virus Genes 59, 874–877 (2023). https://doi.org/10.1007/s11262-023-02027-6

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