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Spermiogenesis produces the spermatozoa with 9 + ‘1’ and 9 + 0 axonemal pattern in progenetic cestode Diplocotyle olrikii Krabbe, 1874 (Spathebothriidea: Acrobothriidae)

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Abstract

Spermiogenesis in the progenetic spathebothriidean cestode Diplocotyle olrikii has been examined using transmission electron microscopy (TEM) for the first time. Along with the typical features of spermatozoon cytodifferentiation (e.g., the electron-dense material in the apical region of the differentiation zone in the early stage of spermiogenesis, the intercentriolar body which is composed of three electron-dense plates and two electron-lucent zones, the orthogonal development of the two flagella, a flagellar rotation, proximo-distal fusion, the presence of two pairs of electron-dense attachment zones), new for the Eucestoda is detection of the formation of two types of free flagella during spermiogenesis in progenetic D. olrikii, exhibiting either standard 9 + ‘1’ trepaxonematan pattern, or atypical 9 + 0 structure. Various combinations of these two types of flagella resulted in the production of three types of male gametes during spermiogenesis in this spathebothriidean cestode. The first type is represented with the two axonemes of the 9 + ‘1’ structure; the second type exhibits two different axonemes, i.e., one with 9 + ‘1’ and the other of 9 + 0 pattern; and the third type has two axonemes with atypical 9 + 0 structure. The occurrence of three sperm types in progenetic D. olrikii is associated with typical spermiogenesis and has never been described previously in the Platyhelminthes. We suppose that heteromorphism of male gametes in D. olrikii might be linked to progenesis, i.e., the programmed sexual maturation detected during the larval/developmental stage of an organism.

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Acknowledgments

We acknowledge the core facility of the Institute of Parasitology, BC ASCR in České Budějovice, Czech Republic, supported by the MEYS CR (LM2015062 Czech-BioImaging). This research was undertaken within the framework of a joint research projects Nos. AV ČR-16-08 and SAV-18-21 supported by a bilateral agreement on scientific exchange and cooperation signed by the Czech and Slovak Academies of Sciences.

Funding

This study was supported by the Grant Agency of the Slovak Republic VEGA (projects no. 2/0104/16 and 2/0126/20).

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Authors and Affiliations

  1. Institute of Parasitology, Slovak Academy of Sciences, 040 01 Košice, Slovak Republic

    Magdaléna Bruňanská

  2. Faculty of Science, Pavol Jozef Šafárik University, 040 01, Košice, Slovak Republic

    Martina Matoušková & Renáta Jasinská

  3. Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05, České Budějovice, Czech Republic

    Jana Nebesářová

  4. Faculty of Science, University of South Bohemia, 370 05, České Budějovice, Czech Republic

    Jana Nebesářová

  5. I.D. Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, Borok, Yaroslavl, Yaroslavl Province, 152 742, Russia

    Larisa G. Poddubnaya

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  1. Magdaléna Bruňanská
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  2. Martina Matoušková
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Correspondence to Magdaléna Bruňanská.

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Bruňanská, M., Matoušková, M., Jasinská, R. et al. Spermiogenesis produces the spermatozoa with 9 + ‘1’ and 9 + 0 axonemal pattern in progenetic cestode Diplocotyle olrikii Krabbe, 1874 (Spathebothriidea: Acrobothriidae). Parasitol Res 119, 4103–4111 (2020). https://doi.org/10.1007/s00436-020-06862-6

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