Light regimen-induced variability of photosynthetic pigments and UV-B absorbing compounds in Luzula sylvatica from Arcto-Alpine tundra

Miloš Barták; Alla Orekhova; Jakub Nezval; Michal Oravec; Josef Hájek; Vladimír Špunda; Jan Tříska; Michaela Bednaříková; Gabriella Nora Maria Giudici; Radek Pech

doi:10.5817/CPR2020-2-20

Light regimen-induced variability of photosynthetic pigments and UV-B absorbing compounds in Luzula sylvatica from Arcto-Alpine tundra

Vol.10,No.2(2020)

Miloš Barták Alla Orekhova Jakub Nezval Michal Oravec Josef Hájek Vladimír Špunda Jan Tříska Michaela Bednaříková Gabriella Nora Maria Giudici Radek Pech

https://doi.org/10.5817/CPR2020-2-20

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Abstract

The aim of this study was to evaluate the effects of different in situ light regimen on ecophysiological parameters of Luzula sylvatica leaves. Plants of L. sylvatica grown under natural sunny and shade conditions in arcto-alpine tundra were analyzed with respect to their leaf anatomy, content of photosynthetic pigments, UV absorbing compounds and phenanthrenoid compounds. Relationship between chlorophyll concentrations (Chl_a+b) and SPAD values was determined for sun and shade leaves measured repeatedly within summer and autumn seasons 2019 and 2020. Pooled data showed curvilinear Chl_a+b to SPAD relationship with the highest Chl_a+b and SPAD values found for shade leaves. Sun leaves had higher UV-B absorbing compounds contents than shade ones. The HPLC-DAD analysis revealed significant amount of soluble flavonoids in Luzula sylvatica leaves, amongst others the flavone-luteolin and its derivatives (e.g. tentatively identified luteolin-methyl-glucoside and luteolin-glucoside). The accumulation of luteolin based compounds in sun acclimated leaves is also plausible explanation for the higher antioxidant activity determined in sun leaf extraxts. Such response of flavonoid metabolism may help L.S. to cope with excessive-light stress through UV-attenuation mechanism and ROS scavanging. Additionally, phenanthrenoid compounds contents in L. sylvatica leaves were determined. Altogether, 9 phenanthrenoid compounds were identified by HPLC-HRMS. Their content was markedly different (up to the factor of 5) between sun and shade leaves of L.sylvatica.

Keywords:
Juncaceae; SPAD values; chlorophyll; carotenoids; phenanthrenoids; sun leaves; shade leaves

References

Agati, G., Biricolti, S., Guidi, L., Ferrini, F., Fini, A. and Tattini, M. (2011): The biosynthesis of flavonoids is enhanced similarly by UV radiation and root zone salinity in L. vulgare leaves. Journal of Plant Physiology, 168: 204-212. Caudle, K. L., Johnson, L. C., Baer, S. G. and Maricle, B. B. (2014): A comparison of seasonal foliar chlorophyll change among ecotypes and cultivars of Andropogon gerardii (Poaceae) by using nondestructive and destructive methods. Photosynthetica, 52 (4): 511-518. Dias, M. C., Figueiredo, C., Pinto, D. C. G. A., Freitas, H., Santos, C. and Silva, A. M. S. (2019): Heat shock and UV-B episodes modulate olive leaves lipophilic and phenolic metabolite profiles. Industrial Crops and Products, 133: 269-275. Demmig-Adams, B., Adams, W. W. III (1992): Carotenoid composition in sun and shade leaves of plants with different life forms. Plant Cell and Environment, 15: 411-419 Dong, T., Shang, J., Chen, J. M., Liu, J., Qian, B., Ma, B., Morrison, M. J., Zhang, C., Liu, Y., Shi, Y., Pan, H. and Zhou, G. (2019): Assessment of portable chlorophyll meters for measuring crop leaf chlorophyll concentration. Remote Sensing, 11(22): 2706. Errecart, P. M., Agnusdei, M. G., Lattanzi, F. A. and Marino, M. A. (2012): Leaf nitrogen concentration and chlorophyll meter readings as predictors of tall fescue nitrogen nutrition status. Field Crops Research, 129: 46-58. Gáborčík, N. (2003): Relationship between contents of chlorophyll (a+b) (SPAD values) and nitrogen of some temperate grasses. Photosynthetica, 41 (2): 285-287. Gainche, M., Ripoche, I., Senejoux, F., Cholet, J., Ogeron, C., Decombat, C., Danton, O., Delort, L., Vareille-Delarbre, M., Berry, A., Vermerie, M., Fraisse, D., Felgines, C., Ranouille, E., Berthon, J.-Y., Priam, J., Saunier, E., Tourrette, A., Troin, Y., Caldefie-Chezet, F. and Chalard, P. (2020): Anti-inflammatory and cytotoxic potential of new phenanthrenoids from Luzula sylvatica. Molecules, 25: 2372. doi:10.3390/molecules25102372. Holub, P., Klem, K., Linder, S. and Urban, O. (2019): Distinct seasonal dynamics of responses to elevated CO2 in two understorey grass species differing in shade tolerance. Ecology and Evolution, 9: 13663-13677. Huang, W., Zhang, S.-B. and Hu, H. (2014): Sun leaves up-regulate the photorespiratory path way to maintain a high rate of CO2 assimilation in tobacco. Frontiers in Plant Science, 5: 688. Kučera, J., Zmrhalová, M., Shaw, B., Košnar, J., Plášek, V. and Váňa, J. (2009): Bryoflora of selected localities of the Hrubý Jeseník Mts summit regions. Časopis Slezské zemské muzeum (A), 58: 115-167. Křížek, M., Krause, D., Uxa, T., Engel, Z., Treml, V. and Traczyk, A. (2019): Patterned ground above the alpine timberline in the High Sudetes, Central Europe. Journal of Maps, 15: 563-569. Lichtenthaler, H. K., Ač, A., Marek, M. V., Kalina, J. and Urban, O. (2007): Differences in pigment composition, photosynthetic rates and chlorophyll fluorescence images of sun and shade leaves of four tree species. Plant Physiology and Biochemistry, 45(8): 577-588. Lin, F. F., Qiu, L. F., Deng, J. S., Shi, Y. Y., Chen, L. S. and Wang, K. (2010): Investigation of SPAD meter-based indices for estimating rice nitrogen status. Computers and Electronics in Agriculture, 715: 560-565. Markwell, J., Osterman, J. C. and Mitchell, J. L. (1995): Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Research, 46: 467-472. Mathur, S., Jain, L. and Jajoo, A. (2018): Photosynthetic efficiency in sun and shade plants. Photosynthetica, 56(1): 354-365. Mielke, M. S., Schaffer, B. and Li, C. (2010): Use of a SPAD meter to estimate chlorophyll content in Eugenia uniflora L. leaves as affected by contrasting light environments and soil flooding. Photosynthetica, 48(3): 332-338. Lichtenthaler, H. K., Wellburn, A. R. (1983): Determinations of total carotenoids and chlorophyllsaandbof leafextracts in different solvents. Biochemical Society Transactions, 11: 591-592. Netto, A. T., Campostrini, E., de Oliveira, Gonҫalves, J., Bressan-Smith, R. E. (2005): Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves. Scientia Horticulturae, 104: 199-209. Newsham, K. K. (2003): UV-B radiation arising from stratospheric ozone depletion influences the pigmentation of the Antarctic moss Andreaea regularis. Oecologia, 135: 327-331. Pandey, S., Kushwaha, R. (2005): Leaf anatomy and photosynthetic acclimation in Valeriana jatamansi L. grown under high and low irradiance. Photosynthetica, 43(1): 85-90. Parry, C,. J. Blonquist, J. M. Jr., Bugbee B. (2014): In situ measurement of leaf chlorophyll concentration: analysis of the optical/absolute relationship. Plant, Cell and Environment, 37: 2508-2520. Sarijeva, G., Knapp, M. and Lichtenthaler, H. K., (2007): Differences in photosynthetic activity, chlorophyll and carotenoid levels, and in chlorophyll fluorescence parameters in green sun and shade leaves of Ginkgo and Fagus. Journal of Plant Physiology, 164: 950-955. Šenfeldr, M., Treml, V. (2020): Which generative reproduction characteristics determine successful establishment of subalpine dwarf shrub Pinus mugo? Journal of Vegetation Science, 31: 403-415. Terashima, I., Miyazawa, S.-I. and Hanba Y. T. (2001): Why are Sun Leaves Thicker than Shade Leaves? Consideration based on Analyses of CO2, Diffusion in the Leaf. Journal of Plant Research, 114: 93-105. Tóth, B., Chang, F.-R., Hwang, T.-L., Szappanos, Á., Mándi, A., Hunyadi, A., Kurtán, T., Jakab, G., Hohmann, J. and Vasas, A. (2017): Screening of Luzula species native to the Carpathian Basin for anti-inflammatory activity and bioactivity-guided isolation of compounds from Luzula luzuloides (Lam.) Dandy & Wilmott. Fitoterapia, 116: 131-138. Uddling, J., Gelang-Alfredsson, J., Piikki, K. and Pleijel, H. (2007): Evaluating the relationship between leaf chlorophyll concentration and SPAD-502 chlorophyll meter readings. Photosynthesis Research, 91: 37-46. Williams, C. A., Harborne, J. B. (1975): Luteolin and daphnetin derivatives in the Juncaceae and their systematic signifikance. Biochemical Systematics and Ecology, 3: 181-190. Wu, J. W., Su, Y., Wang, J. H., He, Q., Qiu, Q., Ma, J. W. and Li, J. Y. (2018): Morphological and physiological acclimation of Catalpa bungei plantlets to different light conditions Photosynthetica, 56(2): 537-548. Xiong, D., Chen, J., Tingting, Y., Gao, W., Ling, X., Li, Y., Shaobing P. and Huang, J. (2015): SPAD-based leaf nitrogen estimation is impacted by environmental factors and crop leaf characteristics. Scientific Reports, 5(1): 13389. Ziedler, M., Duchoslav, M., Banaš, M. and Lešková, M. (2012): Impacts of introduced dwarf pine (Pinus mugo) on the diversity and composition of alpine vegetation. Community Ecology, 13: 213-220. Ziedler, M., Hertlová, B., Banaš, M. and Zahradník, D. (2018): Vegetation shift after a clear-cut of non-native dwarf pine (Pinus mugo). Biologia, 73: 113-119. Web sources / Other sources [1] Public weather data for Praděd, the Jeseniky mountains (non-commertial use), www.meteoblue.com (Meteoblue AG, Basel, Switzerland)

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