Folia Parasitologica 70:001 (2023) | DOI: 10.14411/fp.2023.001

Calicophoron daubneyi (Dinnik, 1962) (Digenea) in beef and dairy cattle in the Czech Republic: prevalence and drug efficacy

Anna Baštýřová Brutovská ORCID...1, 3, Pavlína Vogalová1, 3, Michael Rost ORCID...1, Bohumil Sak ORCID...2, Martin Kváč ORCID...1, 2
1 Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Czech Republic;
2 Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, České Budějovice, Czech Republic;
3 These authors contributed equally

A total of 1,724 beef and 2,941 dairy cattle older than one year from 66 beef and 67 dairy farms in the Czech Republic were examined for the presence of rumen and liver fluke eggs in 2019-2022. Out of 227 positive animals, all were positive for paramphistome and five for fasciolid eggs. Molecular analysis of the ITS2 rDNA revealed the presence of Calicophoron daubneyi (Dinnik, 1962) and Fasciola hepatica Linnaeus, 1758. Faecal egg count (FEC) showed low infection intensity (12 EPG) in animals infected with F. hepatica and high variability in C. daubneyi infections (2-589 EPG). Efficacy of oxyclozanide, albendazole, ivermectin, and closantel against C. daubneyi infection was evaluated at eight beef cattle herds. Faecal samples were collected from all positive animals at 0 and 21days post-treatment. Based on FEC, albendazole, ivermectin and closantel reduced the number of C. daubneyi eggs shed by 0-9.9%, with no effect on the number of infected animals. The use of oxyclozanide on two beef farms showed 100% efficacy against C. daubneyi and F. hepatica. Follow-up examination 5-6 months after drug application showed reinfection of most animals with C. daubneyi, but the FEC was significantly lower. The finding of four dairy cows infected with C. daubneyi housed in a stable without pasture suggests the possibility of the infection being introduced through roughage.

Keywords: PCR, faecal egg counts, albendazole, ivermectin, oxyclozanide, closantel

Received: June 2, 2022; Revised: August 3, 2022; Accepted: August 29, 2022; Published online: January 2, 2023  Show citation

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Baštýřová Brutovská, A., Vogalová, P., Rost, M., Sak, B., & Kváč, M. (2023). Calicophoron daubneyi (Dinnik, 1962) (Digenea) in beef and dairy cattle in the Czech Republic: prevalence and drug efficacy. Folia Parasitologica, 70, Article 2023.001. https://doi.org/10.14411/fp.2023.001
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