Plant Soil Environ., 2022, 68(5):213-222 | DOI: 10.17221/65/2022-PSE

Arsenic-induced response in roots of arsenic-hyperaccumulator fern and soil enzymatic activity changesOriginal Paper

Veronika Zemanová1,2, Daniela Pavlíková1, Milan Novák1, Petre I. Dobrev3, Tomáš Matoušek4, Václav Motyka3, Milan Pavlík ORCID...*,1,2
1 Department of Agro-Environmental Chemistry and Plant Nutrition, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic
2 Isotope Laboratory, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic
3 Laboratory of Hormonal Regulations in Plants, Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic
4 Department of Trace Element Analysis, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic

In a pot experiment, arsenic-hyperaccumulating Pteris cretica cv. Albo-lineata plant ferns were cultivated and exposed to low and high doses of arsenate (20 and 100 mg As/kg, respectively) for six months. Physiological and morphological changes of roots, as well as changes in soil quality of the root zone and bulk soil (water-soluble fraction of elements and activity of soil enzymes), were determined. The results showed that the accumulation of inorganic As, mainly in the form of As3+, did not significantly affect the yield of roots, but caused changes in root morphology (deformation of root cell walls due to lignification) and metabolism (decrease of auxin indole-3-acetic acid and 2-oxoindole-3-acetic acid contents). Although the soil quality results varied according to the As dose, there was a clear difference between the root zone and the bulk soil. The activities of enzymes in the root zone were greater that those in the bulk soil. The results showed a significant influence of the high dose of As (100 mg As/kg), which decreased the activity of arylsulfatase, nitrate reductase, and urease in the root zone, while a decrease in acid phosphatase and nitrate reductase was observed in the bulk soil. The water-soluble fractions of As, organic nitrogen, nitrate nitrogen and organic carbon were significantly affected by the high dose of As.

Keywords: phytohormone; contamination; manganese; metalloid; risk, toxic element

Published: May 15, 2022  Show citation

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Zemanová V, Pavlíková D, Novák M, Dobrev PI, Matoušek T, Motyka V, Pavlík M. Arsenic-induced response in roots of arsenic-hyperaccumulator fern and soil enzymatic activity changes. Plant Soil Environ.. 2022;68(5):213-222. doi: 10.17221/65/2022-PSE.
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