Abstract
This study reconstructs Middle and Late Neolithic dietary practices in the area of the today Czech Republic and Lower Austria with a help of complementary evidence of stable isotope and dental microwear analysis. From a total of 171 humans, carbon and nitrogen isotopic values were measured in bone collagen of 146 individuals (accompanied by 64 animals) while 113 individuals were included into buccal dental microwear analysis. The samples were divided into two newly established chronological phases: Neolithic B (4900–4000 BC) and Neolithic C (3800–3400 BC) based on radiocarbon data modelling. Isotopic results show that the Neolithic diet was of terrestrial origin with a dominant plant component. A small but statistically significant shift in human carbon isotopic values to a higher δ13C was observed during the Neolithic C, probably reflecting an underlying change in plant growing conditions. Dental microwear results suggest a shift in adult diet and/or food preparation techniques between the Neolithic B and C, which, however, was not reflected in either the carbon or nitrogen isotopic values. The positive correlations between nitrogen isotopic values and the dental microwear variables (NV, XV, XT and NV/NT) observed in the adult sample suggest that meat rather than milk was the dominant source of animal protein, or that food enriched in 15N was processed specifically. Also, as both methods offer a snapshot of different periods of an individual’s life, the presence of a significant correlation may imply highly repetitive dietary behaviour during their lifetime.
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Data availability
The authors declare that the data supporting the findings of this study are available within the article and its supplementary information files.
References
Ambrose SH, Butler BM, Hanson DB, Hunter-Anderson RL, Krueger HW (1997) Stable isotopic analysis of human diet in the Marianas Archipelago, Western Pacific. Am J Phys Anthropol 104:343–361. https://doi.org/10.1002/(SICI)1096-8644(199711)104:3%3c343::AID-AJPA5%3e3.0.CO;2-W
Ambrose SH, Norr L (1993) Experimental evidence for the relationship of the carbon isotope ratios of whole diet and dietary protein to those of bone collagen and carbonate. In Lambert JB, Grupe G (eds) Prehistoric human bone: Archaeology at the molecular level. Springer, Berlin, pp 1–37. https://doi.org/10.1007/978-3-662-02894-0_1
Arens NC, Jahren AH, Amundson R (2000) Can C3 plants faithfully record the carbon isotopic composition of atmospheric carbon dioxide? Paleobiology 26:137–164
Beaumont J, Atkins E-C, Buckberry J, Haydock H, Horne P, Howcroft R, Mackenzie K, Montgomery J (2018) Comparing apples and oranges: why infant bone collagen may not reflect dietary intake in the same way as dentine collagen. Am J Phys Anthropol 167:524–540. https://doi.org/10.1002/ajpa.23682
Bickle P (2018) Stable isotopes and dynamic diets: the Mesolithic-Neolithic dietary transition in terrestrial central Europe. J Archaeol Scie Rep 22:444–451. https://doi.org/10.1016/j.jasrep.2018.09.017
Bickle P, Whittle A (2013) The first farmers of Central Europe: diversity in LBK lifeways. Oxbow Books, Oxford
Bickle P, Bentley RA, Dočkalová M, Fibiger L, Griffiths S, Hamilton J, Hedges REM, Hofmann D, Mateiciucová I, Whittle A (2014) Early Neolithic lifeways in Moravia and Western Slovakia: comparing archaeological, osteological and isotopic data from cemetery and settlement burials of the Linearbandkeramik (LBK). Anthropologie 52:35–72
Bocherens H, Drucker D (2003) Trophic level isotopic enrichment of carbon and nitrogen in bone collagen: case studies from recent and ancient terrestrial ecosystems. Int J Osteoarchaeol 13:46–53. https://doi.org/10.1002/oa.662
Bocherens H, Polet C, Toussaint M (2007) Palaeodiet of Mesolithic and Neolithic populations of Meuse Basin (Belgium): evidence from stable isotopes. J Archaeol Sci 34:10–27. https://doi.org/10.1016/j.jas.2006.03.009
Bocherens H (1992) Biogéochimie isotopique (13C, 15N, 18O) et paléontologie des vertébrés: applications à l'étude des réseaux trophiques révolus et des paléoenvironnements. Dissertation,Université Paris VI
Bogaard A, Heaton THE, Poulton P, Merbach I (2007) The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices. J Archaeol Sci 34:335–343. https://doi.org/10.1016/j.jas.2006.04.009
Bonsall C, Lennon R, McSweeney K, Stewart C, Harkness D, Boroneanţ V, Bartosiewicz L, Payton R, Chapman J (1997) Mesolithic and early Neolithic in the Iron Gates: a palaeodietary perspective. J Eur Archaeol 5:50–92. https://doi.org/10.1179/096576697800703575
Bronk Ramsey C (2017) Methods for summarizing radiocarbon datasets. Radiocarbon 59:1809–1833. https://doi.org/10.1017/RDC.2017.108
Brown TA, Nelson DE, Vogel JS, Southon JR (1988) Improved collagen extraction by modified Longin method. Radiocarbon 30:171–177. https://doi.org/10.1017/S0033822200044118
Brychova V, Roffet-Salque M, Pavlu I, Kyselka J, Kyjakova P, Filip V, Světlík I, Evershed RP (2021) Animal exploitation and pottery use during the early LBK phases of the Neolithic site of Bylany (Czech Republic) tracked through lipid residue analysis. Quat Int 574:91–101. https://doi.org/10.1016/j.quaint.2020.10.045
Budd C, Bogucki P, Lillie M, Grygiel R, Lorkiewicz W, Schulting R (2020) All things bright: copper grave goods and diet at the Neolithic site of Osłonki, Poland. Antiquity 94:932–947. https://doi.org/10.15184/aqy.2020.102
Burger J, Kirchner M, Bramanti B, Haak W, Thomas MG (2007) Absence of the lactase-persistence-associated allele in early Neolithic Europeans. Proc Natl Acad Sci 104:3736–3741. https://doi.org/10.1073/pnas.0607187104
Cox G, Sealy J (1997) Investigating identity and life histories: isotopic analysis and historical documentation of slave skeletons found on the Cape Town foreshore, South Africa. Int J Hist Archaeol 1:207–224. https://doi.org/10.1023/A:1027349115474
DeNiro MJ (1985) Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 317:806–809. https://doi.org/10.1038/317806a0
DeNiro MJ, Epstein S (1978) Influence of diet on the distribution of carbon isotopes in animals. Geochim Cosmochim Acta 42:495–506. https://doi.org/10.1016/0016-7037(78)90199-0
DeNiro MJ, Epstein S (1981) Influence of diet on the distribution of nitrogen isotopes in animals. Geochim Cosmochim Acta 45:341–351. https://doi.org/10.1016/0016-7037(81)90244-1
Diefendorf AF, Mueller KE, Wing SL, Koch PL, Freeman KH (2010) Global patterns in leaf 13C discrimination and implications for studies of past and future climate. Proc Natl Acad Sci 107:5738–5743. https://doi.org/10.1073/pnas.0910513107
Doppler T, Gerling C, Heyd V, Knipper C, Kuhn T, Lehmann MF, Pike AWG, Schibler J (2017) Landscape opening and herding strategies: carbon isotope analyses of herbivore bone collagen from the Neolithic and Bronze Age lakeshore site of Zurich-Mozartstrasse, Switzerland. Quat Int 436:18–28. https://doi.org/10.1016/j.quaint.2015.09.007
Dreslerová D, Kočár P (2013) Trends in cereal cultivation in the Czech Republic from the Neolithic to the Migration period (5500 b.c.–a.d. 580). Veg Hist Archaeobot 22:257–268. https://doi.org/10.1007/s00334-012-0377-8
Drucker DG, Bridault A, Hobson KA, Szuma E, Bocherens H (2008) Can carbon-13 in large herbivores reflect the canopy effect in temperate and boreal ecosystems? Evidence from modern and ancient ungulates. Palaeogeogr Palaeoclimatol Palaeoecol 266:69–82. https://doi.org/10.1016/j.palaeo.2008.03.020
Drucker DG, Hobson KA, Ouellet J-P, Courtois R (2010) Influence of forage preferences and habitat use on 13C and 15N abundance in wild caribou (Rangifer tarandus caribou) and moose (Alces alces) from Canada. Isot Environ Health Stud 46:107–121. https://doi.org/10.1080/10256010903388410
Dufour E, Bocherens H, Mariotti A (1999) Palaeodietary implications of isotopic variability in Eurasian lacustrine fish. J Archaeol Sci 26:617–627. https://doi.org/10.1006/jasc.1998.0379
Dürrwächter C, Craig OE, Collins MJ, Burger J, Alt KW (2006) Beyond the grave: variability in Neolithic diets in Southern Germany? J Archaeoll Sci 33:39–48. https://doi.org/10.1016/j.jas.2005.06.009
El-Zaatari S (2010) Occlusal microwear texture analysis and the diets of historical/prehistoric hunter-gatherers. Int J Osteoarchaeol 20:67–87. https://doi.org/10.1002/oa.1027
Estebaranz F, Martínez LM, Galbany J, Turbón D, Pérez-Pérez A (2009) Testing hypotheses of dietary reconstruction from buccal dental microwear in Australopithecus afarensis. J Hum Evol 57:739–750. https://doi.org/10.1016/j.jhevol.2009.07.007
Estebaranz F, Galbany i Casals J, Martinez Martinez LM, Turbón D, Martínez Pérez-Pérez A (2012) Buccal dental microwear analyses support greater specialization in consumption of hard foodstuffs for Australopithecus anamensis. J Anthropol Sci 90:163–185. https://doi.org/10.4436/jass.90006
Fahy GE, Deter C, Pitfield R, Miszkiewicz JJ, Mahoney P (2017) Bone deep: variation in stable isotope ratios and histomorphometric measurements of bone remodelling within adult humans. J Archaeol Sci 87:10–16. https://doi.org/10.1016/j.jas.2017.09.009
Farquhar GD, Ehleringer JR, Hubick KT (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–537. https://doi.org/10.1146/annurev.pp.40.060189.002443
Fernandes R, Fernandes R, Nadeau M-J, Grootes PM (2012) Macronutrient-based model for dietary carbon routing in bone collagen and bioapatite. Archaeol Anthropol Sci 4:291–301. https://doi.org/10.1007/s12520-012-0102-7
Fernandes R, Millard AR, Brabec M, Nadeau M-J, Grootes P (2014) Food Reconstruction Using Isotopic Transferred Signals (FRUITS): a Bayesian model for diet reconstruction. Plos One 9:e87436. https://doi.org/10.1371/journal.pone.0087436
Fernandes R, Grootes P, Nadeau M-J, Nehlich O (2015) Quantitative diet reconstruction of a Neolithic population using a Bayesian mixing model (FRUITS): the case study of Ostorf (Germany). Am J Phys Anthropol 158:325–340. https://doi.org/10.1002/ajpa.22788
Fiorentino G, Ferrio JP, Bogaard A, Araus JL, Riehl S (2015) Stable isotopes in archaeobotanical research. Veg Hist Archaeobot 24:215–227. https://doi.org/10.1007/s00334-014-0492-9
Fox CL, Pérez-Pérez A (1993) The diet of the Neanderthal Child Gibraltar 2 (Devil’s Tower) through the study of the vestibular striation pattern. J Hum Evol 24:29–41. https://doi.org/10.1006/jhev.1993.1004
Fraser RA, Bogaard A, Schäfer M, Arbogast R, Heaton THE (2013) Integrating botanical, faunal and human stable carbon and nitrogen isotope values to reconstruct land use and palaeodiet at LBK Vaihingen an der Enz, Baden-Württemberg. World Archaeol 45:492–517. https://doi.org/10.1080/00438243.2013.820649
Fuller BT, Fuller JL, Harris DA, Hedges REM (2006) Detection of breastfeeding and weaning in modern human infants with carbon and nitrogen stable isotope ratios. Am J Phys Anthropol 129:279–293. https://doi.org/10.1002/ajpa.20249
Fuller BT, Müldner G, Neer WV, Ervynck A, Richards MP (2012) Carbon and nitrogen stable isotope ratio analysis of freshwater, brackish and marine fish from Belgian archaeological sites (1st and 2nd millennium AD). J Anal at Spectrom 27:807–820. https://doi.org/10.1039/C2JA10366D
Furholt M (2021) Mobility and social change: understanding the European Neolithic period after the archaeogenetic revolution. J Archaeol Res 29:481–535. https://doi.org/10.1007/s10814-020-09153-x
Galbany J, Martínez Martínez LM, Péréz-Perez A (2004) Tooth replication techniques, SEM imaging and microwear analysis in primates: methodological obstacles. Anthropologie 42:5–12
Galbany J, Martínez LM, López-Amor HM, Espurz V, Hiraldo O, Romero A, de Juan J, Pérez-Pérez A (2005) Error rates in buccal-dental microwear quantification using scanning electron microscopy. Scanning 27:23–29. https://doi.org/10.1002/sca.4950270105
Gillis RE, Kovačiková L, Bréhard S, Guthmann E, Vostrovská I, Nohálová H, Arbogast R-M, Domboróczki L, Pechtl J, Anders A, Marciniak A, Tresset A, Vigne J-D (2017) The evolution of dual meat and milk cattle husbandry in Linearbandkeramik societies. Proc Royal Soc B 284:20170905. https://doi.org/10.1098/rspb.2017.0905
Goude G, Salazar-García DC, Power RC, Terrom J, Rivollat M, Deguilloux M-F, Pemonge M-H, Le Bailly M, Andre G, Coutelas A, Hauzeur A (2019) A multidisciplinary approach to Neolithic life reconstruction. J Archaeol Method Theory 26:537–560. https://doi.org/10.1007/s10816-018-9379-x
Greenfield HJ (2010) The secondary products revolution: the past, the present and the future. World Archaeol 42:29–54. https://doi.org/10.1080/00438240903429722
Williams FL, Schmidt CW, Droke JL (2020) Dietary reconstruction of Late Neolithic farmers of the Belgian Meuse basin using dental microwear texture analysis. Anthropologie Et Prehistoire 129(2018):73–86
Griffiths S (2013) Radiocarbon dates from Nitra, Schwetzingen and Vedrovice. In: Bickle P, Whittle A (eds) The first farmers of central Europe: diversity in LBK lifeways. Oxbow, Oxford, pp 443–458
Guiry EJ (2012) Dogs as analogs in stable isotope-based human paleodietary reconstructions: a review and considerations for future use. J Archaeol Method Theory 19:351–376. https://doi.org/10.1007/s10816-011-9118-z
Halcrow SE (2019) Early Europeans bottle-fed babies with animal milk. Nature 574:182–183. https://doi.org/10.1038/d41586-019-02805-z
Hammann S, Cramp LJE (2021) Ancient foodomics: analytical strategies for reconstruction of palaeodiets and implications for human health. In: Cifuentes S (ed) Comprehensive Foodomics. Elsevier, Cambridge, pp 197–210
Hammond C, O’Connor T (2013) Pig diet in medieval York: carbon and nitrogen stable isotopes. Archaeol Anthropol Sci 5:123–127. https://doi.org/10.1007/s12520-013-0123-x
Hedges REM, Reynard LM (2007) Nitrogen isotopes and the trophic level of humans in archaeology. J Archaeol Sci 34:1240–1251. https://doi.org/10.1016/j.jas.2006.10.015
Hedges REM, Clement JG, Thomas CDL, O’Connell TC (2007) Collagen turnover in the adult femoral mid-shaft: modeled from anthropogenic radiocarbon tracer measurements. Am J Phys Anthropol 133:808–816. https://doi.org/10.1002/ajpa.20598
Hernando R, Gamarra B, McCall A et al (2021) Integrating buccal and occlusal dental microwear with isotope analyses for a complete paleodietary reconstruction of Holocene populations from Hungary. Sci Rep 11:7034. https://doi.org/10.1038/s41598-021-86369-x
Herrscher E, Bras-Goude GL (2010) Southern French Neolithic populations: isotopic evidence for regional specificities in environment and diet. Am J Phys Anthropol 141:259–272. https://doi.org/10.1002/ajpa.21141
Hrnčíř V, Vondrovský V, Květina P (2020) Post-marital residence patterns in LBK: comparison of different models. J Anthropol Archaeol 59:101190. https://doi.org/10.1016/j.jaa.2020.101190
Jarošová I (2007) Dental buccal microwear of the population from Dolní Věstonice, Czech Republic. Anthropologie 45:71–80
Jarošová I (2008) Dietary inferences using buccal microwear analysis on the LBK population from Vedrovice, Czech Republic. Anthropologie 46:175–184
Jarošová I, Tvrdý Z (2017) Diet and diversity of early farmers in Neolithic period (LBK): buccal dental microwear and stable isotopic analysis at Vedrovice (Czech Republic) and Nitra - Horné Krškany (Slowakia). Anthropologie 55:353–384
Jarošová I, Pérez-Pérez A, Dočkalová M, Drozdová E, Turbón D (2006) Buccal dental microwear as a dietary indicator in the Iron Age population from Son Real, Spain. Anthropologie 44:139–150
Jarošová I, Vávra J, Jiřík J, Horáková M (2016) Buccal dental microwear of a Barbarian population from Prague-Zličín. IANSA 7:55–70
Katzenberg MA, Weber A (1999) Stable isotope ecology and palaeodiet in the Lake Baikal region of Siberia. J Archaeol Sci 26:651–659. https://doi.org/10.1006/jasc.1998.0382
Kaupová S, Velemínský P, Herrscher E, Sládek V, Macháček J, Poláček L, Brůžek J (2018) Diet in transitory society: isotopic analysis of medieval population of Central Europe (ninth–eleventh century AD, Czech Republic). Archaeol Anthropol Sci 10:923–942. https://doi.org/10.1007/s12520-016-0427-8
Kaupová S, Velemínský P, Stránská P, Bravermanová M, Frolíková D, Tomková K, Frolík J (2019) Dukes, elites, and commoners: dietary reconstruction of the early medieval population of Bohemia (9th–11th Century AD, Czech Republic). Archaeol Anthropol Sci 11:1887–1909. https://doi.org/10.1007/s12520-018-0640-8
Kovačiková L, Brehard S, Šumberová R, Balasse M, Tresset A (2012) The new insights into the subsistence and early farming from neolithic settlements in Central Europe: the archaeozoological evidence from the Czech Republic. Archaeofauna 21:71–97
Kučera L, Peška J, Fojtík P, Barták P, Kučerová P, Pavelka J, Komárková V, Beneš J, Polcerová L, Králík M, Bednář P (2019) First direct evidence of broomcorn millet (Panicum miliaceum) in Central Europe. Archaeol Anthropol Sci 11:4221–4227. https://doi.org/10.1007/s12520-019-00798-4
Lalueza C, Pérez-Pérez A, Turbón D (1993) Microscopic study of the Banyoles mandible (Girona, Spain): diet, cultural activity and toothpick use. J Hum Evol 24:281–300. https://doi.org/10.1006/jhev.1993.1022
Lalueza C, Péréz-Perez A, Turbón D (1996) Dietary inferences through buccal microwear analysis of Middle and Upper Pleistocene human fossils. Am J Phys Anthropol 100:367–387. https://doi.org/10.1002/(SICI)1096-8644(199607)100:3%3c367::AID-AJPA5%3e3.0.CO;2-R
Lam YM (1994) Isotopic evidence for change in dietary patterns during the Baikal Neolithic. Curr Anthropol 35:185–190. https://doi.org/10.1086/204256
Lee-Thorp JA (2008) On isotopes and old bones. Archaeometry 50:925–950. https://doi.org/10.1111/j.1475-4754.2008.00441.x
Longin R (1971) New method of collagen extraction for radiocarbon dating. Nature 230:241–242. https://doi.org/10.1038/230241a0
Lubell D, Jackes M, Schwarcz H, Knyf M, Meiklejohn C (1994) The Mesolithic-Neolithic transition in Portugal: isotopic and dental evidence of diet. J Archaeol Sci 21:201–216. https://doi.org/10.1006/jasc.1994.1022
Mahoney P (2006) Dental microwear from Natufian hunter-gatherers and early Neolithic farmers: comparisons within and between samples. Am J Phys Anthropol 130:308–319. https://doi.org/10.1002/ajpa.20311
Marciniak A (2011) The secondary products revolution: empirical evidence and its current zooarchaeological critique. J World Prehist 24:117–130. https://doi.org/10.1007/s10963-011-9045-7
Martínez LM, Pérez-Pérez A (2004) Post-mortem wear as indicator of taphonomic processes affecting enamel surfaces of Hominin teeth from Laetoli and Olduvai (Tanzania): implications to dietary interpretations. Anthropologie 42:37–42
Masclans A, Bickle P, Hamon C (2021) Sexual inequalities in the early Neolithic? : exploring relationships between sexes/genders at the cemetery of Vedrovice using use-wear analysis, diet and mobility. J Archaeol Method Theory 28:232–273
Mathieson I, Lazaridis I, Rohland N et al (2015) Genome-wide patterns of selection in 230 ancient Eurasians. Nature 528:499–503. https://doi.org/10.1038/nature16152
McLaughlin TR (2007) Diet in Mesolithic Europe: new evidence from dental microwear. Internet Archaeology 22. https://doi.org/10.11141/ia.22.1
Merah O, Deléens E, Teulat B, Monneveux P (2002) Association between yield and carbon isotope discrimination value in different organs of durum wheat under drought. J Agron Crop Sci 188:426–434. https://doi.org/10.1046/j.1439-037X.2002.00594.x
Metcalfe SP, Macdonald JZ, R A, (2017) Best practices for calibrating and reporting stable isotope measurements in archaeology. J Archaeol Sci Rep 13:609–616. https://doi.org/10.1016/j.jasrep.2017.05.007
Milner N, Craig OE, Bailey GN, Pedersen K, Andersen SH (2004) Something fishy in the Neolithic? A re-evaluation of stable isotope analysis of Mesolithic and Neolithic coastal populations. Antiquity 78:9–22. https://doi.org/10.1017/S0003598X00092887
Minagawa M, Wada E (1984) Stepwise enrichment of 15N along food chains: further evidence and the relation between δ15N and animal age. Geochim Cosmochim Acta 48:1135–1140. https://doi.org/10.1016/0016-7037(84)90204-7
Mnich B, Mueller-Bieniek A, Nowak M, Wilczyński J, Pospuła S, Szostek K (2020) Terrestrial diet in prehistoric human groups from southern Poland based on human, faunal and botanical stable isotope evidence. J Archaeol Sci Rep 32:102382. https://doi.org/10.1016/j.jasrep.2020.102382
Molleson T, Jones K, Jones S (1993) Dietary change and the effects of food preparation on microwear patterns in the Late Neolithic of abu Hureyra, northern Syria. J Hum Evol 24:455–468. https://doi.org/10.1006/jhev.1993.1031
Mueller-Bieniek A, Nowak M, Styring A, Lityńska-Zając M, Moskal-del Hoyo M, Sojka A, Paszko B, Tunia K, Bogaard A (2019) Spatial and temporal patterns in Neolithic and Bronze Age agriculture in Poland based on the stable carbon and nitrogen isotopic composition of cereal grains. J Archaeol Sci Rep 27:101993. https://doi.org/10.1016/j.jasrep.2019.101993
Münster A, Knipper C, Oelze VM, Nicklisch N, Stecher M, Schlenker B, Ganslmeier R, Fragata M, Friederich S, Dresely V, Hubensack V, Brandt G, Döhle H-J, Vach W, Schwarz R, Metzner-Nebelsick C, Meller H, Alt KW (2018) 4000 years of human dietary evolution in central Germany, from the first farmers to the first elites. Plos One 13:e0194862. https://doi.org/10.1371/journal.pone.0194862
Neustupný E, Dobeš M, Turek J, Zápotocký M (2013) The prehistory of Bohemia. 3 The Eneolithic. Archeologický ústav AV ČR Praha, Praha
Nystrom P (2008) Dental microwear signatures of an early LBK population from Vedrovice, the Czech Republic. Anthropologie 46:161–174
O’Connell TC, Kneale CJ, Tasevska N, Kuhnle GGC (2012) The diet-body offset in human nitrogen isotopic values: a controlled dietary study. Am J Phys Anthropol 149:426–434. https://doi.org/10.1002/ajpa.22140
Oelze VM, Siebert A, Nicklisch N, Meller H, Dresely V, Alt KW (2011) Early Neolithic diet and animal husbandry: stable isotope evidence from three Linearbandkeramik (LBK) sites in Central Germany. J Archaeol Sci 38:270–279. https://doi.org/10.1016/j.jas.2010.08.027
Organ JM, Teaford MF, Larsen CS (2005) Dietary inferences from dental occlusal microwear at mission San Luis de Apalachee. Am J Phys Anthropol 128:801–811. https://doi.org/10.1002/ajpa.20277
Pajdla P, Trampota F (2021) Neolithic settlements in Central Europe: data from the project ‘Lifestyle as an unintentional identity in the Neolithic.’ Journal of Open Archaeology Data 9:13. https://doi.org/10.5334/joad.88
Parnell AC, Inger R, Bearhop S, Jackson AL (2010) Source partitioning using stable isotopes: coping with too much variation. Plos One 5:e9672. https://doi.org/10.1371/journal.pone.0009672
Pérez-Pérez A (2004) Why buccal microwear? Anthropologie 42:1–4
Pérez-Pérez A, Lalueza C, Turbón D (1994) Intraindividual and intragroup variability of buccal tooth striation pattern. Am J Phys Anthropol 94:175–187. https://doi.org/10.1002/ajpa.1330940203
Pérez-Pérez A, Bermúdez De Castro JM, Arsuaga JL (1999) Nonocclusal dental microwear analysis of 300,000-year-old Homo heilderbergensis Teeth from Sima de los Huesos (Sierra de Atapuerca, Spain). Am J Phys Anthropol 108:433–457. https://doi.org/10.1002/(SICI)1096-8644(199904)108:4%3c433::AID-AJPA5%3e3.0.CO;2-5
Pérez-Pérez A, Espurz V, Bermúdez de Castro JM, de Lumley MA, Turbón D (2003) Non-occlusal dental microwear variability in a sample of Middle and Late Pleistocene human populations from Europe and the Near East. J Hum Evol 44:497–513. https://doi.org/10.1016/S0047-2484(03)00030-7
Pérez-Pérez A, Martínez LM, Gómez M, Estebaranz-Sánchez F, Romero A (2018) Correlations among dietary proxies in African fossil hominins: dental buccal microwear, occlusal textures and 13C stable isotope. J Archaeol Sci Rep 22:384–391. https://doi.org/10.1016/j.jasrep.2018.03.013
Pérez-Pérez A (1990) Evolución de la dieta en Cataluña y Baleares desde el Paleolítico hasta le edad Media a partir de restos esqueléticos. Dissertation, Universitat de Barcelona
Pinilla Pérez B, Romero A, Pérez-Pérez A (2011) Age-related variability in buccal dental-microwear in Middle and Upper Pleistocene human populations. Anthropol Rew 74:25–37. https://doi.org/10.2478/v10044-010-0005-0
Polo-Cerdá M, Romero A, Casabó J, De Juan J (2007) The Bronze Age burials from Cova Dels Blaus (Vall d′Uixó, Castelló, Spain): an approach to palaeodietary reconstruction through dental pathology, occlusal wear and buccal microwear patterns. Homo 58:297–307. https://doi.org/10.1016/j.jchb.2006.10.005
Regenye J, Bánffy E, Demján P, Ebert J, Osztás A, Bronk Ramsey C, Dunbar E, Friedrich R, Bayliss A, Beavan N, Gaydarska B, Whittle A (2020) Narratives for Lengyel funerary practice. Bericht Der Romish-Germanischen Kommission 97:5–81
Reitsema LJ, Kozłowski T, Makowiecki D (2013) Human–environment interactions in medieval Poland: a perspective from the analysis of faunal stable isotope ratios. J Archaeol Sci 40:3636–3646. https://doi.org/10.1016/j.jas.2013.04.015
Richards MP, Hedges REM (1999) A Neolithic revolution? New evidence of diet in the British Neolithic. Antiquity 73:891–897. https://doi.org/10.1017/S0003598X00065649
Richards MP, Pearson JA, Molleson TI, Russell N, Martin L (2003a) Stable isotope evidence of diet at Neolithic Çatalhöyük, Turkey. J Archaeol Sci 30:67–76. https://doi.org/10.1006/jasc.2001.0825
Richards MP, Price TD, Koch E (2003b) Mesolithic and Neolithic subsistence in Denmark: new stable isotope data. Curr Anthropol 44:288–295. https://doi.org/10.1086/367971
Richards MP, Montgomery J, Nehlich O, Grimes V (2008) Isotopic analysis of humans and animals from Vedrovice. Anthropologie 46:185–194
Roffet-Salque M, Dunne J, Altoft DT, Casanova E, Cramp LJE, Smyth J, Whelton HL, Evershed RP (2017) From the inside out: upscaling organic residue analyses of archaeological ceramics. J ArchaeolSci Rep 16:627–640. https://doi.org/10.1016/j.jasrep.2016.04.005
Romero A, De Juan J (2007) Iintra- and interpopulation human buccal tooth surface microwear analysis: inferences about diet and formation processes. Anthropologie 45:61–70
Romero A, Martínez-Ruiz N, de Juan J (2004) Non-occlusal dental microwear in a Bronze-Age human sample from East Spain. Anthropologie 42:65–70
Romero A, Galbany J, De Juan J, Pérez-Pérez A (2012) Brief communication: short- and long-term in vivo human buccal–dental microwear turnover. Am J Phys Anthropol 148:467–472. https://doi.org/10.1002/ajpa.22054
Romero A, Ramírez-Rozzi FV, Juan JD, Pérez-Pérez A (2013) Diet-related buccal dental microwear patterns in Central African Pygmy foragers and Bantu-speaking farmer and pastoralist populations. Plos One 8:e84804. https://doi.org/10.1371/journal.pone.0084804
Salazar-García DC, Romero A, García-Borja P, Subirà ME, Richards MP (2016) A combined dietary approach using isotope and dental buccal-microwear analysis of human remains from the Neolithic, Roman and Medieval periods from the archaeological site of Tossal de les Basses (Alicante, Spain). J Archaeol Sci Rep 6:610–619. https://doi.org/10.1016/j.jasrep.2016.03.002
Schmidt CW, Remy A, Van Sessen R, Willman J, Krueger K, Scott R, Mahoney P, Beach J, McKinley J, D’Anastasio R, Chiu L, Buzon M, De Gregory JR, Sheridan S, Eng J, Watson J, Klaus H, Da-Gloria P, Wilson J, Stone A, Sereno P, Droke J, Perash R, Stojanowski C, Herrmann N (2019) Dental microwear texture analysis of Homo sapiens sapiens: foragers, farmers, and pastoralists. Am J Phys Anthropol 169:207–226. https://doi.org/10.1002/ajpa.23815
Schoeninger MJ, DeNiro MJ (1984) Nitrogen and carbon isotopic composition of bone collagen from marine and terrestrial animals. Geochim Cosmochim Acta 48:625–639. https://doi.org/10.1016/0016-7037(84)90091-7
Shennan S (2018) The first farmers of Europe: an evolutionary perspective. Cambridge University Press, Cambridge
Sherratt A (1981) Plough and pastoralism: aspects of the secondary products revolution. In: Hodder I, Isaac G, Hammond N (eds) Pattern of the Past: Studies in honour of David Clarke. Cambridge University Press, Cambridge, pp 261–305
Siebke I, Furtwängler A, Steuri N, Hafner A, Ramstein M, Krause J, Lösch S (2020) Crops vs. animals: regional differences in subsistence strategies of Swiss Neolithic farmers revealed by stable isotopes. Archaeol Anthropol Sci 12:235. https://doi.org/10.1007/s12520-020-01122-1
Tieszen LL (1991) Natural variations in the carbon isotope values of plants: implications for archaeology, ecology, and paleoecology. J Archaeol Sci 18:227–248. https://doi.org/10.1016/0305-4403(91)90063-U
Timpson A, Colledge S, Crema E, Edinborough K, Kerig T, Manning K, Thomas MG, Shennan S (2014) Reconstructing regional population fluctuations in the European Neolithic using radiocarbon dates: a new case-study using an improved method. J Archaeol Sci 52:549–557. https://doi.org/10.1016/j.jas.2014.08.011
Trampota F, Květina P (2020) How do they fit together? A case study of Neolithic pottery typology and radiocarbon chronology. Archeologicke Rozhledy 72:163–193. https://doi.org/10.35686/AR.2020.6
van Klinken GJ (1999) Bone collagen quality indicators for palaeodietary and radiocarbon measurements. J Archaeol Sci 26:687–695. https://doi.org/10.1006/jasc.1998.0385
Vostrovská I (2018) Těšetice-Kyjovice – komunitní areál prvních zemědělců. Dissertation, Masarykova univerzita
Webb EC, Honch NV, Dunn PJH, Linderholm A, Eriksson G, Lidén K, Evershed RP (2018) Compound-specific amino acid isotopic proxies for distinguishing between terrestrial and aquatic resource consumption. Archaeol Anthropol Sci 10:1–18. https://doi.org/10.1007/s12520-015-0309-5
Whittle A, Bentley RA, Bickle P, Dočkalová M, Fibiger L, Hamilton J, Hedges REM, Mateiciucová I, Pavúk J (2013) Moravia and western Slovakia. In: Bickle P, Whittle A (eds) The first farmers in central Europe: Diversity in LBK lifeways. Oxbow books, Oxford, pp 101–158
Acknowledgements
We thank David Gaul for the English language editing. Many thanks are due to Lenka Kovačiková and Gabriela Dreslerová for species determination of animal bones. We are also grateful to researchers who provided anthropological and zooarchaeological material or valuable advice: Petra Beran-Cimbůrková, Zdeněk Beneš, Hana Brzobohatá, Pavel Burgert, Lukáš Hlubek, Jiří Kala, Petr Kos, Marek Lečbych, Kerstin Liden, David Parma, Michal Přichystal, Klára Rybářová, Petr Stadler, Jitka Stříšková, Lukáš Šín, Miroslav Šmíd, Peter Tóth, Karin Wiltschke-Schrota.
Funding
This study was supported financially by Czech Science Foundation (“Lifestyle as an unintentional identity in the Neolithic”. Project Id: 19-16304S) and Ministry of Culture of the Czech Republic (DKRVO 2019–2023/7.I.e, 00023272). OP RDE, MEYS, under the project “Ultra-trace isotope research in social and environmental studies using accelerator mass spectrometry”, Reg. No. CZ.02.1.01/0.0/0.0/16_019/0000728.
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All authors contributed to the study conception and design. Material preparation were performed by František Trampota, Jaroslav Řídký, Christine Neugebauer-Maresch and Petr Květina. Data collection and analysis were performed by Sylva Drtikolová Kaupová, Jarmila Bíšková, Ivana Jarošová, Dalia A. Pokutta, Zdeněk Tvrdý, Zdeněk Vytlačil, Václav Hrnčíř and František Trampota. The first draft of the manuscript was written by Sylva Drtikolová Kaupová and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Drtikolová Kaupová, S., Jarošová, I., Bíšková, J. et al. The diet of settled Neolithic farmers of east-central Europe: isotopic and dental microwear evidence. Archaeol Anthropol Sci 15, 21 (2023). https://doi.org/10.1007/s12520-023-01720-9
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DOI: https://doi.org/10.1007/s12520-023-01720-9