Skip to main content
SpringerLink
Log in

Plant Cytogenetics and Cytogenomics

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Tony Heitkam

    1. Institute of Botany, TU Dresden, Dresden, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Sònia Garcia

    1. Botanical Institute of Barcelona, Barcelona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2672)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 229.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book USD 299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (33 protocols)

  1. Front Matter

    Pages i-xviii
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Plant Cytogenetics: From Chromosomes to Cytogenomics

      • Trude Schwarzacher, Qing Liu, J. S. (Pat) Heslop-Harrison
      Pages 3-21

  3. Sizing the Nucleus: Methods for Genome Size and Ploidy Level Estimation

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants

      • João Loureiro, Martin Čertner, Magdalena Lučanová, Elwira Sliwinska, Filip Kolář, Jaroslav Doležel et al.
      Pages 25-64

    3. Nuclear DNA Content Estimation of Seaweed by Fluorimetry Analysis

      • Rafael P. Martín-Martín, Noemí Salvador-Soler, Jordi Rull Lluch, Amelia Gómez Garreta
      Pages 65-77

    4. K-Mer-Based Genome Size Estimation in Theory and Practice

      • Uljana Hesse
      Pages 79-113

    5. A Bioinformatic Pipeline to Estimate Ploidy Level from Target Capture Sequence Data Obtained from Herbarium Specimens

      • Juan Viruel, Oriane Hidalgo, Lisa Pokorny, Félix Forest, Barbara Gravendeel, Paul Wilkin et al.
      Pages 115-126

  4. Getting to the Chromosomes: Methods for Chromosome Fixation, Preparation, and Manipulation

    1. Front Matter

      Pages 127-127
      Download chapter PDF

    2. Nitrous Oxide-Induced Metaphase Arrest: A Technique for Somatic Chromosome Analysis

      • Patrice S. Albert, James A. Birchler
      Pages 129-139

    3. Preparation of Mitotic Chromosomes with the Squash Technique

      • Alexis J. Maravilla, Marcela Rosato, Josep A. Rosselló
      Pages 141-149

    4. Preparation of Mitotic Chromosomes with the Dropping Technique

      • Nicola Schmidt, Beatrice Weber, Jessica Klekar, Susan Liedtke, Sarah Breitenbach, Tony Heitkam
      Pages 151-162

    5. Laser Capture Microdissection: From Genomes to Chromosomes, from Complex Tissue to Single-Cell Analysis

      • Tomáš Janíček, Roman Hobza, Vojtěch Hudzieczek
      Pages 163-175

    6. Flow Cytometric Analysis and Sorting of Plant Chromosomes

      • Petr Cápal, Mahmoud Said, István Molnár, Jaroslav Doležel
      Pages 177-200

  5. Putting on Color: Banding and Staining Techniques

    1. Front Matter

      Pages 201-201
      Download chapter PDF

    2. C-Banding of Plant Chromosomes

      • Adam J. Lukaszewski
      Pages 203-214

    3. CMA/DAPI Banding of Plant Chromosomes

      • Ana Emília Barros e Silva, Marcelo Guerra
      Pages 215-224

    4. Silver Nitrate Staining of Nucleolar Organizer Regions (Ag-NORs) in Plant Chromosomes

      • Claudio Palma-Rojas, Pedro Jara-Seguel, Cristian Araya-Jaime
      Pages 225-231

    5. Chromatin Immunostaining of Plant Nuclei

      • Nobuko Ohmido, Aqwin Polosoro
      Pages 233-244

  6. Labeling DNA: In Situ Hybridization and Other Methods Using Fluorescent Labels

    1. Front Matter

      Pages 245-245
      Download chapter PDF
Back to top

Keywords

About this book

This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.


Editors and Affiliations

  • Institute of Botany, TU Dresden, Dresden, Germany

    Tony Heitkam

  • Botanical Institute of Barcelona, Barcelona, Spain

    Sònia Garcia

Bibliographic Information

  • Book Title: Plant Cytogenetics and Cytogenomics

  • Book Subtitle: Methods and Protocols

  • Editors: Tony Heitkam, Sònia Garcia

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3226-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-3225-3Published: 20 June 2023

  • Softcover ISBN: 978-1-0716-3228-4Due: 04 August 2023

  • eBook ISBN: 978-1-0716-3226-0Published: 19 June 2023

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 568

  • Number of Illustrations: 14 b/w illustrations, 106 illustrations in colour

  • Topics: Plant Genetics and Genomics, Biological Techniques

Publish with us

Policies and ethics

Back to top

Search

Navigation