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Innovative technique for the direct determination of proteins in calcified aortic valves

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Abstract

Aortal valve mineralization very frequently causes a genesis of aortic stenosis, which is the most often surgically treated heart disease. Hydroxyapatite deposits have been identified as one of the causes leading to the loss of elasticity of the aortic valves. It is known that phosphates/calcium is accumulated in valve tissues during mineralization, but the mechanism of this process remains unclear. The work is focused mainly on the study of protein composition of mineralized aortic valves by nano-liquid chromatography electrospray ionization in a quadrupole orthogonal acceleration time-of-flight mass spectrometry. New methodological approach based on direct enzymatic digestion of proteins contained in hydroxyapatite deposits was developed for the study of pathological processes connected with osteogenesis. Our objectives were to simplify the traditional analytical protocols of sample preparation and to analyze the organic components of the explanted aortic valves for significant degenerative aortic stenosis. The study of aortic valve mineralization on the molecular level should contribute to understanding this process, which should consequently lead to effective prevention as well as to new ways of treatment of this grave disease.

The photo of explanted calcification of human aortal valve.

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Acknowledgments

This study was financially supported by Specific University Research (MSMT no. 20/2013) and Project Operational Programme Prague Competitiveness (OPPC) CZ.2.16/3.100/22197.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Institute of Chemical Technology, Technická 5, 166 28, Prague, Czech Republic

    Lucie Coufalova, Stepanka Kuckova, Martina Velcovska & Radovan Hynek

  2. Institute of Theoretical and Applied Mechanics, Academy of Science of the Czech Republic, Prosecká 76, 190 00, Prague, Czech Republic

    Antonin Zeman

  3. Department of Cardiology, University Hospital Pilsen, alej Svobody 80, 304 60, Pilsen, Czech Republic

    Michal Smid

  4. Institute of Rock Structure and Mechanics, Academy of Science of the Czech Republic, V Holešovičkách 41, 182 09, Prague, Czech Republic

    Martina Havelcova

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  1. Lucie Coufalova
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  2. Stepanka Kuckova
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  3. Martina Velcovska
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  4. Antonin Zeman
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  5. Michal Smid
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  7. Radovan Hynek
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Correspondence to Lucie Coufalova.

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Coufalova, L., Kuckova, S., Velcovska, M. et al. Innovative technique for the direct determination of proteins in calcified aortic valves. Anal Bioanal Chem 405, 8781–8787 (2013). https://doi.org/10.1007/s00216-013-7306-2

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