Properties of Lipid Models of Lung Surfactant Containing Cholesterol and Oxidized Lipids: A Mixed Experimental and Computational Study

0524869 - ÚFCH JH 2021 RIV US eng J - Článek v odborném periodiku
Olžyńska, Agnieszka - Delcroix, Pauline - Dolejsova, T. - Krzaczek, K. - Korchowiec, B. - Czogalla, A. - Cwiklik, Lukasz
Properties of Lipid Models of Lung Surfactant Containing Cholesterol and Oxidized Lipids: A Mixed Experimental and Computational Study.
Langmuir. Roč. 36, č. 4 (2020), s. 1023-1033. ISSN 0743-7463
Grant CEP: GA ČR(CZ) GA17-06792S
Institucionální podpora: RVO:61388955
Klíčová slova: pulmonary surfactant * dynamics * membranes * simulations * monolayers * calcium
Obor OECD: Physical chemistry
Impakt faktor: 3.882, rok: 2020
Způsob publikování: Omezený přístup

We introduce and study a multicomponent lipid film mimicking lipid composition of the human lung surfactant. It consists of phospholipids with various lipid headgroups and tail saturation. Furthermore, it includes cholesterol and oxidized lipids. Langmuir trough and fluorescence microscopy experiments are combined with fully atomistic molecular dynamics simulations. The considered lipid mixtures form complex interfacial films with properties modulated by lateral compression. Cholesterol laterally condenses, and oxidized lipids laterally expand the films. Both types of molecules increase film miscibility. Oxidized lipids also alter the lipid-water interface enhancing film hydration. This effect can be partially reversed by cholesterol. Regarding presentation of different chemical moieties toward the aqueous subphase, the zwitterionic phosphatidylcholine groups dominate at the lipid-water interface, while both the negatively charged phosphatidylglycerol and hydroxyl group of cholesterol are less exposed. The investigated synthetic lipid-only mimic of the lung surfactant may serve as a basis for further studies involving nonlipid pulmonary surfactant components.
Trvalý link: http://hdl.handle.net/11104/0309114